中华丝膜菌子实体发生规律及菌丝生长培养基研究OA

To protect the Cortinarius sinensis in the Helan mountains of Ningxia and promote its artificial domestication,two methods of field observation and laboratory experiment observation were adopted for the investigation.The field observation results showed that this fungus occurred in the pure Picea crassifolia forest(occasionally mixed with Populus davidian,etc.)on the semi-shaded and semi-sunny slopes above an altitude of 2,100 meters in the Helan mountains,with a canopy density of 0.58 to 0.66,a pH value of 7.5 to 8.0,soil being gray-brown soil or alpine meadow soil,surface litter thickness of 1.4 to 2.0 cm,and dense mycelium in the 0 to 10 cm soil layer.It was prone to occur when the daily average temperature was 14 to 25℃,the ground temperature was 8 to 18℃,and the annual precipitation was more than 500 mm.The peak occurrence period was from late August to early September.The continuous 2 to 3 days of high temperature after a rain shower was one of the key conditions for a large number of occurrences.Meanwhile,the composition of soil humus carbon and nitrogen nutrients that the fungus relied on was determined.Based on the determination results of soil humus nutrients,orthogonal experiments were designed using potato extract solution(as a carbon source),peptone(as a nitrogen source),MgSO₄,and KH₂PO₄,and indoor cultivation experiments were carried out using an artificial climate chamber to simulate the wild habitat.The results showed that the culture medium with 150 g/L potato extract solution,3 g/L peptone,2 g/L MgSO₄,and 2 g/L KH₂PO₄ could promote the growth of mycelia when inoculated with the gill of this fungus,providing support for the protection and domestication of this fungus.