蓖麻全基因组WRKY基因家族的鉴定及表达分析OA
Identification and expression analysis of the WRKY gene family in the whole genome of Ricinus communis
[目的]鉴定蓖麻 WRKY 转录因子家族成员,为进一步探究蓖麻 WRKY 基因家族成员的功能、进化及分子育种奠定基础.[方法]运用生物信息学方法对蓖麻 WRKY转录因子家族成员蛋白质的理化性质、二级结构、保守基序、系统进化树、基因表达差异及启动子顺式作用元件进行分析.[结果]蓖麻 WRKY 基因家族中共鉴定出58 个基因.理化性质和二级结构分析显示,蓖麻 WRKY转录因子家族大多数为碱性蛋白和亲水性蛋白,二级结构以无规则卷曲为主.蛋白质保守基序分析显示,Motif1、Motif2 所占的比例较高;系统进化树可将其分为 9 个亚族,大部分亚族中都包含蓖麻和拟南芥 WRKY 蛋白.基因表达差异分析发现,其中多数 W RKY 基因都至少在蓖麻发育中的一个时期表达;荧光定量分析结果显示,3 个基因在叶中的表达量均低于在花和第 50 天种子中的表达量,RcWRKY31 在叶中几乎不表达,且RcWRKY57在第 25 天种子中表达量相对较低,即同一基因家族在蓖麻叶、花、种子中的表达存在一定差异;启动子分析表明,WRKY 基因家族启动子区含有 12 个响应生物胁迫和非生物胁迫的顺式作用元件.[结论]蓖麻中共检测到 58 个WRKY 基因,能够参与复杂的抗逆反应以及生长发育调控过程,帮助蓖麻应对逆境胁迫及其自身生长带来的不利影响.
[Objective]To identify the members of WRKY transcription factor family of Ricinus communis,and provide a basis for further studies of the functions,evolution,and molecular breeding of the WRKY gene family members of R.communis.[Methods]Bioinformatics approaches were applied to analyze the physicochemical properties,secondary structures,conserved motifs,phylogenetic tree analysis,gene expression differences,and promoter cis-acting elements of proteins of WRKY transcription factor family members of R.communis.[Results]A total of 58 genes were identified in the WRKY gene family of R.communis.Analyses of physicochemical properties and secondary structure indicated that most proteins in WRKY transcription factor gene family were basic and hydrophilic proteins,and the secondary structure consists mainly of random coils.Protein conserved motif analysis showed that Motif1 and Motif2 accounted for a relatively high proportion.Phylogenetic tree analysis classified these proteins into nine subfamilies,and most subfamilies contained WRKY proteins of both R.communis and Arabidopsis thaliana.Gene expression difference analysis revealed that most of WRKY genes were expressed at least at one stage during R.communis development.Quantitative real-time analysis results indicated that the expression levels of three genes in leaves were lower than those in flowers and in seeds at 50 d.RcWRKY31 was almost not expressed in leaves,and RcWRKY57 showed relatively low expression in seeds at 25 d.These findings indicated that members of the same gene family exhibit certain differences in expression among R.communis leaves,flowers,and seeds.Promoter analysis showed that the promoter regions of WRKY gene family contain 12 cis-acting elements responsive to both biotic and abiotic stresses.[Conclusion]A total of 58 WRKY genes are identified in R.communis.These genes are involved in complex stress response and growth and development regulatory processes,thereby helping R.communis to cope with the adverse effects from environmental stresses and its own growth.
张春玲;狄建军;韩雯毓;刘庆秀;王冬艳;曲毅鹏;张斯琴;何智彪
通辽市农牧业科学院,内蒙古 通辽 028015内蒙古自治区蓖麻育种与综合利用重点实验室/蓖麻育种国家民委重点实验室,内蒙古 通辽 028000||内蒙古民族大学,内蒙古 通辽 028000||内蒙古自治区高校蓖麻产业工程技术研究中心/内蒙古自治区蓖麻育种重点实验室,内蒙古 通辽 028000通辽市农牧业科学院,内蒙古 通辽 028015通辽市科尔沁区农业技术推广中心,内蒙古 通辽 028000内蒙古民族大学,内蒙古 通辽 028000通辽市农牧业科学院,内蒙古 通辽 028015通辽市科尔沁区农业技术推广中心,内蒙古 通辽 028000通辽市农牧业科学院,内蒙古 通辽 028015||通辽市农业技术推广中心,内蒙古 通辽 028000||通辽市园林管理局,内蒙古 通辽 028000
农业科技
蓖麻WRKY基因转录因子生物信息学
Ricinus communisWRKY geneTranscription factorBioinformatics
《北方农业学报》 2026 (2)
11-24,14
中央引导地方科技发展专项(YDZX202021)通辽市科技计划项目"通辽市农作物新品种选育及示范推广"通辽市财政支持项目"种业振兴及新品种研发"
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