白藜芦醇及其衍生物对断奶仔猪生长性能、肝脏损伤及内质网应激的影响OA

[Objectives]The aim of this study was to investigate the effects of dietary supplementation with resveratrol and its derivative pterostilbene on growth performance,hepatic damage,and endoplasmic reticulum stress(ERS)in weaned piglets.[Methods]A total of 300 21-day-old weaned piglets Duroc×Landrace×Large White were randomly assigned to five treatment groups,with six replicates per group and ten piglets per replicate.The control group was fed a basal diet,while the resveratrol group was fed a diet supplemented with 250 mg•kg-1 resveratrol.The pterostilbene groups were fed diets supplemented with 100,250,and 500 mg•kg-1 pterostilbene.The experiment lasted for 21 days,during which growth performance was recorded.At the end of the trial,one piglet from each replicate was randomly selected for slaughter and sampling.[Results]Supplementation with 250 and 500 mg•kg-1 pterostilbene significantly improved feed efficiency in piglets during the 3-week post-weaning period(P<0.05),and the 250 mg•kg-1 pterostilbene group showed a tendency to increase average daily gain compared with the control group(P＝0.067).Supplementation with 250 mg•kg-1 resveratrol partially reduced liver cell apoptosis in weaned piglets(P＝0.098),whereas 250 and 500 mg•kg-1 pterostilbene significantly decreased the levels of apoptotic liver cells(P<0.05).Compared with the control group,the resveratrol group exhibited significantly lower expression levels of activating transcription factor 6(ATF6)and glucose-regulated protein 78(GRP78)in the liver(P<0.05).The 250 and 500 mg•kg-1 pterostilbene groups showed significantly reduced transcription levels of ERS-related genes,including protein kinase R-like ER kinase,ATF6,GRP78,and C/EBP homologous protein compared with the control group(P<0.05),while sirtuin 1 activity was significantly increased(P<0.05).Additionally,supplementation with 250 mg•kg-1 pterostilbene significantly increased calnexin expression levels in the liver(P<0.05).[Conclusions]Pterostilbene was more effective than resveratrol in improving growth performance,alleviating hepatic damage,and regulating ER stress in weaned piglets,with an optimal level of 250 mg•kg-1.