锰纳米颗粒的制备及其增强免疫功能研究OA

[Objectives]The paper aimed to study the activation of manganese nanoparticles(Mnnan)on antigen presenting cells and the immune enhancing efficacy of the H9N2 vaccine.[Methods]This research prepared nanoparticles with chicken serum albumin and manganese chloride,and then identified manganese nanoparticles by nanoparticle correlation characterization method.Finally,manganese nanoparticles were used as immunopotentiator.The activation of antigen presenting cells and the immune enhancing efficacy of the H9N2 vaccine were analyzed.[Results]The size of manganese nanoparticles was about 50 nm,and the shape was like a cube.It showed good stability after being placed at 4℃for 60 days.X-ray diffraction(XRD)data showed that the prepared manganese nanoparticles were manganic tetroxide.In vitro experiments showed that manganese nanoparticles could activate the NF-κB signaling pathway of RAW-Blue cells and induce the expression of IFN-γ,IFN-β,IL-12 and TNF-α mRNA.Manganese nanoparticles could increase the specific hemagglutination inhibition antibody level after H9N2 virus immunization in chickens.[Conclusions]Manganese nanoparticles were successfully prepared in this study,which proved that they had good immunostimulating activity in vivo and in vitro.The results of this study laid a foundation for the research and development of nanoscale immunopotentiator for veterinary use.