微酸性电解水对梨果生炭疽菌的杀菌机制和防控效果研究OA

[Objectives]This study aimed to identify the main pathogen of pear anthracnose and explore the inhibitory mechanism of slightly acidic electrolyzed water(SAEW)on Colletotrichum fructicola and its control effect on postharvest pear fruit anthracnose.[Methods]Tissue isolation,morphology and molecular biology methods were used to identify the pathogenic bacteria of pear anthracnose.Based on this,the mycelium and spores were treated with SAEW at different concentrations to further investigate its antifungal mechanism.[Results]Through morphology and molecular biology,the main pathogen of pear anthracnose was identified as C.fructicola TJ-21.SAEWexhibited significant inhibitory effects on C.fructicola TJ-21,with its efficacy positively correlated with the effective chlorine concentration.The treatment with 150 mg•L-1 SAEW for 5 min completely inhibited mycelial growth,while the treatment with 200 mg•L-1 SAEW for 5 min significantly reduced spore germination rate,disrupted the cell membrane permeability,and caused leakage of cell contents.Furthermore,SAEW showed significant control effects on pear anthracnose,with 200 mg•L-1 SAEW treatment for 5 min significantly inhibiting the expansion of disease lesions.[Conclusions]C.fructicola was one of the major pathogens causing pear anthracnose.SAEW,as a novel green fungicide,exerted its antimicrobial effect by inhibiting the growth of C.fructicola,increasing cell membrane permeability,and disrupting the cell membrane.