茄科植物PMI基因进化研究及辣椒CaPMI基因克隆和表达分析OA

[Objectives]This study aimed to identify the phosphomannose isomerase(PMI)gene in Solanaceae and Capsicum annuum,investigate its evolutionary processes,and systematically analyze the expression regulatory network of the CaPMI gene in C.annuum under abiotic stress and phytohormone treatments.[Methods]The characteristics of PMI gene family were analyzed by bioinformatics and molecular cloning techniques,and the transcriptome data were analyzed to clarify the expression characteristics of Solanaceae PMI in organ development and fruit maturation.The expression pattern of pepper under multiple stresses was detected by RT-qPCR.[Results]PMI gene was strongly purified and selected(Ka/Ks<1)during the evolutionary process,forming three independent evolutionary branches,and specific preservation occurred in different species.Three CaPMI genes were identified in the pepper genome,and all the promoter regions of Solanaceae PMI genes were enriched with elements related to growth and development,stress response and hormone regulation,but there were significant differences in the number and combination patterns.The CaPMI1(1 299 bp/432 aa)and CaPMI2(1 326 bp/441 aa)genes were cloned from a pepper cDNA library,and their encoded proteins were acidic(pI 4.83-4.91).Stress response analysis revealed that CaPMI1 was significantly activated under cold,heat,and gibberellin(GA3)treatments,whereas CaPMI2 and CaPMI3 responded rapidly to cold,heat,drought,GA3,and salicylic acid(SA)stresses.[Conclusions]This study revealed the conservation and functional innovation of Solanaceae PMI genes in evolution,and the CaPMI gene family formed a stress-response module of CaPMI1,CaPMI2 and CaPMI3 through neo-functionalization,which enhanced the adaptability of pepper to complex environmental pressures and provided genetic resources and theoretical support for the creation of stress-resistant germplasm in pepper.