龙葵外泌体通过诱导凋亡抑制人肝癌HepG2细胞的增殖、迁移与侵袭OA

Aim To investigate whether solanum nigrum exosomes inhibit the proliferation,migration and invasion of human hepatocellular carcinoma HepG2 cells by inducing apoptosis.Methods Solanum nigrum exosomes were extracted and identified for their morphology and particle size characteristics.HepG2 cells were cultured in vitro and divided into blank group,cisplatin group,low-dose group(8.045 mg/L solanum nigrum exosomes),medium-dose group(16.090 mg/L solanum nigrum exosomes)and high-dose group(32.180 mg/L solanum nigrum exosomes).The cell proliferation inhibition rate was detected by CCK-8 assay,and cell apoptosis was detected by TUNEL assay.The migration and invasion abilities of cells were evaluated by plate colony formation,Transwell cham-ber and wound healing assays.The expression levels of apoptosis-related genes and proteins were analyzed by real-time quantitative PCR and Western blotting.Results Solanum nigrum exosomes significantly inhibited cell proliferation in a concentration-and time-de-pendent manner(P<0.01).Compared with the blank group,the apoptosis rate,and the mRNA and protein expression levels of Bax,caspase-3,caspase-8 and caspase-9 were increased in all solanum nigrum exosome dose groups and the cisplatin group,while the colony formation ability,wound healing rate,number of migrated cells,number of invaded cells,and the mRNA and protein expression levels of Bcl-2 were decreased(P<0.01).Compared with the cisplatin group,the changes of the above indicators in the medium-and high-dose solanum nigrum exosome groups were more significant(P<0.01),with a concentration-dependent effect.Conclusion Solanum nigrum exosomes may inhibit the proliferation,migration,and invasion of human hepatocellular carcinoma HepG2 cells by inducing apoptosis.