柠檬醛对两种植物病原菌的抑菌作用探究OA

In this experiment,citral was used as the research object to determine its antifungal effect on Botrytis cinerea and Colletotrichum gloeosporioides.The antifungal mechanism of citral was elucidated by observing the hyphae morphology of plant pathogens,determining the effect of ergosterol content on cell membrane and determining the effect of extracellular conductivity.The results showed that citral exhibited fumigation inhibitory effects on both plant pathogenic fungi.Citral demonstrated stronger inhibitory activity against C.gloeosporioides,with EC50 and EC90 values of 1.488 μL/dish and 5.213 μL/dish,respectively,while those against B.cinerea were 1.492 μL/dish and 3.080 μL/dish.The observation of the antifungal mechanism showed that after citral fumigation,the hyphae at the edge of the colony were sparse,aberred,the surface collapsed,and the protoplasm leaked.After treatment,the content of ergosterol of the two plant pathogens decreased,but the extracellular conductivity increased,indicating that the bioactive components had inhibitory effect on pathogens.It can be seen that citral has the potential to be used as a fungicide.