1种载药水凝胶的制备及抗菌作用研究OA

To address the challenges of bacterial skin wound infections in animals and the poor water solubility and low stability of curcumin,this study aimed to develop a new type of drug-loaded nanogel and evaluate its antibacterial properties.The study utilized the in-situ crosslinking method combining ionic crosslinking and Schiff base reaction,using chitosan microspheres and curcumin-loaded phase-change microspheres as precursors,and successfully prepared curcumin nanogel at a volume ratio of 4∶6.The structure was observed using scanning electron microscopy(SEM),and its antibacterial effect was investigated.The results showed that the curcumin nanogel had a dense structure with uniform pore diameters(approximately 10～100 μm)in a three-dimensional porous structure,and exhibited superior antibacterial activity and persistence compared to free curcumin against the standard strain of Staphylococcus aureus ATCC 29213 and its small colony variant(SCVA 102).The minimum inhibitory concentration(MIC value)was 250 and 250 mg/L,and it maintained a stable antibacterial effect for 72 hours.The study demonstrated that the prepared curcumin nanogel has good sustained-release properties and significant in vitro antibacterial activity,providing a potential new local administration route for treating animal skin wound infections and controlling drug-resistant bacteria.