SlBBX20基因对番茄叶绿素合成的影响OA

Chlorophyll is an essential core pigment in plants,which plays a vital role in driving photosynthesis to provide energy for plant growth and regulating plant coloration.SlBBX20(Solanum lycopersicum B-box zinc finger transcription factor 20)is a key member of the tomato SlBBXs gene family,and participates in carotenoid biosynthesis,photomorphogenesis,anthocyanin accumulation and low-temperature stress response.To clarify whether SlBBX20 regulates chlorophyll metabolism and influences chlorophyll synthesis in tomato,we investigated the SlBBX20 knockout line Cri-BBX20 generated by gene editing.The results showed that the knockout line exhibited pale leaf color and significantly decreased chlorophyll content.Transcriptome sequencing was performed using wild-type A57 and the knockout line.Functional enrichment analysis indicated that differentially expressed genes were mainly enriched in the biosynthesis of amino acids and carbon metabolism pathways.In addition,promoter cis-element prediction was conducted on eight chlorophyll synthesis-related genes,and the promoters of these genes all contained cis-acting elements that can bind to SlBBX20.Collectively,SlBBX20 may modulate the transcription of genes involved in the chlorophyll biosynthesis pathway,thereby regulating chlorophyll biosynthesis in tomato.