短期高糖饮食对哮喘小鼠气道炎症的影响OA
Effects of short-term high-sugar diet on airway inflammation in asthmatic mice
目的:探讨短期高糖饮食通过调节 Th 细胞比例促进哮喘小鼠气道炎症的作用与机制.方法:BALB/c小鼠随机分为对照组、高糖饮食组、哮喘组和高糖哮喘组.对照组和哮喘组给予普通饮水,高糖饮食组和高糖哮喘组给予20%葡萄糖水.哮喘组和高糖哮喘组采用卵清蛋白诱导哮喘模型.HE 染色和PAS 染色观察肺组织病理及杯状细胞增生,瑞氏染色检测嗜酸性粒细胞百分比,流式细胞术分析 Th 细胞比例,实时荧光定量 PCR 检测细胞因子表达.结果:与对照组相比,高糖饮食组气道炎症无明显改变,Th 细胞比例及细胞因子表达无显著差异(P>0.05);哮喘组和高糖哮喘组气道炎症明显,嗜酸性粒细胞及 Th2、Th17 细胞比例明显升高,Th1、Treg 细胞比例明显下降,IL-4、IL-17 mRNA 表达明显升高,TGF-β mRNA 表达明显降低(P 均<0.05),且高糖哮喘组 IFN-γ mRNA 表达明显低于对照组(P<0.05).与哮喘组相比,高糖哮喘组 Th1、Treg 细胞比例明显降低,Th2、Th17 细胞比例明显升高,IL-4、IL-17 mRNA 表达明显升高,TGF-β mRNA 表达明显降低(P 均<0.05).结论:短期高糖饮食可上调哮喘小鼠 Th2、Th17 细胞比例,下调 Th1、Treg 细胞比例,加重气道炎症.
Objective:To investigate the role and mechanism of short-term high-sugar diet in airway inflammation in asthmatic mice by regulating the proportion of Th cells.Methods:BALB/c mice were randomly divided into control group,high-sugar diet group,asthma group and high-sugar asthma group.Mice in control group and asthma group were fed with normal drinking water,while those in high-sugar diet group and high-sugar asthma group were fed with 20%glucose water.Ovalbumin was used to establish asthmatic model in asthma group and high-sugar asthma group.HE and PAS staining were used to observe lung histopathology and goblet cell hyperplasia.Wright's staining was used to detect the percentage of eosinophils.Flow cytometry was used to analyze the proportion of Th cells,and qRT-PCR was used to detect the expression of cytokines.Results:Compared with the control group,airway inflammation in the high-sugar diet group showed no obvious changes,and there was no significant difference in Th cells proportion and cytokine expression(P>0.05).However,when compared to the control group,airway inflammation in both the asthma group and high-sugar asthma group was obvious,the proportions of eosinophils,Th2 and Th17 cells were significantly increased,the proportions of Th1 cells and Treg cells were significantly decreased,the expressions of IL-4 and IL-17 mRNA were significantly up-regulated,and the expression of TGF-β mRNA was significantly down-regulated(all P<0.05).The expression of IFN-γ mRNA in the high-sugar asthma group was significantly lower than that in the control group(P<0.05).Compared with the asthma group,the proportions of Th1 and Treg cells in the high-sugar asthma group were significantly decreased,the proportions of Th2 and Th17 cells were significantly increased,the expressions of IL-4 and IL-17 mRNA were significantly up-regulated,and the expressions of TGF-β mRNA was significantly down-regulated(all P<0.05).Conclusion:Short-term high-sugar diet can up-regulate the proportions of Th2 and Th17 cells,down-regulate the proportions of Th1 and regulatory T cells,and subsequently aggravate airway inflammation in asthmatic mice.
叶济贤;王诏玉;汪雪峰
江苏大学附属医院中心实验室,江苏 镇江 212001常州市儿童医院儿科,江苏 常州 213003江苏大学附属医院中心实验室,江苏 镇江 212001
医药卫生
高糖饮食辅助性T细胞细胞因子哮喘气道炎症免疫调节
high sugar dietT helper cellcytokinesasthmaairway inflammationimmunomodulation
《江苏大学学报（医学版）》 2026 (3)
192-196,5
国家自然科学基金资助项目(81871243)江苏省社会发展项目(BE2017697)江苏省"六大人才高峰"项目(WSN-009)镇江市中医妇科病临床医学研究中心开放课题(SS202204-KFB02)
评论