大黄鱼和黄姑鱼对变形假单胞菌感染的抗病力差异及皮肤转录组分析OA

To investigate the differences in disease resistance between Larimichthys crocea and Nibea albi-flora against Pseudomonas plecoglossicida infection,as well as the immune response dynamics of their skin dur-ing the infection process,both immersion and intraperitoneal injection methods were used to challenge the fish.Skin tissues were collected at 3,24,and 48 h after immersion challenge for transcriptome sequencing analysis.The results showed that N.albiflora exhibited significantly lower morbidity and mortality than L.crocea,indica-ting a stronger resistance to infection.Following immersion challenge,L.crocea began to show disease symptoms and mortality by the third day,with a morbidity rate of 65.3%and mortality rate of 37.5%by the seventh day,respectively,whereas N.albiflora showed no signs of disease or death.After injection challenge,the mor-bidity and mortality rates of L.crocea reached 96.5%and 68.3%by the seventh day,respectively,while those of N.albiflora were significantly lower at 55.5%and 38.3%,respectively.In L.crocea,a strong immune acti-vation was observed as early as 3 h post-infection,with a large number of differentially expressed genes en-riched in inflammatory response pathways such as cytokine-cytokine receptor interaction,IL-17 signaling,and NF-κB signaling,and this high level of immune activation was maintained throughout the infection period.In contrast,N.albiflora exhibited a milder and more regulated immune response,with lower activation of immune-related pathways and a transition toward neuro-regulation and metabolic homeostasis pathways after 24 h.