光生物调控缓解皮肤衰老的效应与机制研究OA

Skin aging is characterized by dermal atrophy,degeneration of elastic fibers,collagen depletion,and attenuated epidermal turnover.Photobiomodulation therapy(PBMT),particularly red light irradiation,has demonstrated promising anti-aging potential;however,its underlying molecular mechanisms remain incompletely elucidated.In this study,we established a natural aging mouse model(18～20 months)to investigate the rejuvenating effects and mechanisms of red light treatment with parameter of 630～635 nm,fluence 8 J/cm2,power density 6.67 mW/cm2,20 min per session for 28 consecutive days.Our results demonstrated that red light significantly increased dermal thickness and enhanced type I collagen deposition in aged mice.Mechanistic investigations revealed that red light promoted collagen synthesis in fibroblasts via activation of the AKT signaling pathway;concurrently,AKT activation stimulated keratinocyte proliferation with elevated Ki67 expression,and differentiation with increased cytokeratin 10 expression,effects that were abrogated by the AKT inhibitor API-2.These findings collectively indicate that red light mediates coordinated dermal-epidermal remodeling through AKT signaling,providing a theoretical foundation for the application of photobiomodulation therapy in the prevention and treatment of skin aging.