蒙药肉豆蔻五味改善心肌缺血大鼠心脑损伤作用机制研究OA

Objective This study investigated the effects of Nutmeg-5 on cardiac function and brain damage in rats with myocardial ischemia.Methods Thirty-two 8-week-old male SD rats were randomly divided into four groups(n=8 each):the control group,the control-treated group,the model group,and the model-treated group.Myocardial ischemia was induced in the model group Control-treated group via ligation of the left anterior descending coronary artery.The model drug group received pre-treatment with Nutmeg-5 extract(0.54 g/kg)one week prior to modeling.Following successful modeling,the model drug group and model drug group continued to receive the same dose of the drug,while the Normal Group and Model Group received an equal volume of carboxymethyl cellulose sodium solution,with continuous intervention for 3 weeks.Body Weight changes and electrocardiograms were monitored in all groups before and after intervention;Cardiac structure and function were assessed using cardiac echocardiography;sensorimotor function and spatial learning and memory abilities were evaluated via the balance beam test and water maze test;levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),acetylcholinesterase(AchE),and glial fibrillary acidic protein(GFAP)in hippocampal tissue were measured using the ELISA method.Results(1)Body Weight:Rats in the model group ex-hibited pseudo-weight gain in the first week post-surgery,followed by a continuous decline in body weight(compared with the control group,P<0.01);body weight showed an upward trend after drug administration.(2)Electrocardiogram:Rats in the model group exhibited significant ST-segment elevation,which largely returned to normal after drug intervention.(3)Echocardiography:Compared with the control group,the model group exhibited thinning of the left ventricular anterior wall,with significantly increased left ventricular end-diastolic volume and left ventricular end-systolic volume(P<0.01)and significantly decreased ejection fractions and fractional shortening(P<0.01);in the model group treated with the drug,all these parameters improved significantly(P<0.01).(4)Behavioral Tests:Compared with the control group,rats in the model group showed significantly prolonged balance beam crossing time and water maze escape latency(P<0.01);compared with the model group,the drug-treated model group exhibited significant reductions in these times(P<0.01).(5)Hippocampal Tissue Factors:Compared with the model group,the levels of IL-1β,TNF-α,AchE,and GFAP in the hippocampal tissue of the model-treated group were significantly reduced(P<0.05,P<0.01).Conclusion Nutmeg-5 significantly improves cardiac function in rats with myocardial ischemia and may alleviate sensory-motor and cognitive behavioral impairments caused by myocardial ischemia by inhibiting hippocampal neuroinflammation,modulating the cholinergic system,and regulating glial cell activation.