蒙药沏其日甘八味片对高脂血症模型金黄地鼠肠道菌群的影响OA

Objective To investigate the regulatory effects of Mongolian medicine Qiqirigan-8 Tablets on intestinal microbiota in hyperlipidemic golden hamsters and to compare its efficacy with that of the traditional pill formulation and formula-deficient preparations.Methods Sixty healthy male golden hamsters were randomly divided into six groups(n=10 per group):the control group,the model group,the Black Borneol-deficient negative control group,Rhei Radix et Rhizoma-deficient negative control group,Qiqirigan-8 Tablets group,and the Qiqirigan-8 Pills group.The golclen hamsters in the control group received a normal diet,while the other groups were fed a high-fat diet for 6 weeks to establish a hyperlipidemic model.After successful modeling,the treatment groups were administered corresponding drugs by gavage:The Black Borneol-deficient negative control group,the Rhei Radix et Rhizoma-deficient negative control group,and the Qiqirigan-8 Tablets group at 36.68 mg/kg,the Qiqirigan-8 Pills group at 104.95 mg/kg,and the control and model groups received equal volumes of 0.5%sodium carboxymethyl-cellulose solution,once daily for 12 weeks.After the last administration,animals were fasted for 12 hours,and blood samples were collected to measure serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)levels.Liver index was calculated.Cecal contents were collected for 16S rRNA gene high-throughput sequencing to analyze gut microbiota diversity,species composition,differential taxa and microbial function.Results Compared with the control group,the model group showed significantly elevated serum TC,TG,LDL-C,liver index,and AST and ALT activities(P<0.01).Intestinal microbiota Chao1,Shannon,and Simpson indices were significantly decreased(P<0.01),the Firmicutes/Bacteroidetes ratio was increased,and the total number of ASVs(2402)and unique ASVs(988)were markedly reduced.Compared with the model group,the Qiqirigan-8 Tablets group exhibited significantly decreased serum TC,TG,LDL-C(P<0.01),reduced liver index(P<0.01),and alleviated the abnormal increase of serum AST and ALT(P<0.01).Intestinal microbiota Chao1,Shannon,and Simpson indices were significantly restored(P<0.01),the Firmicutes/Bacteroidetes ratio was significantly decreased(P<0.05),total ASV number(2933)approached that of the control group(2964),and the microbial community structure recovered to the level of the control group.PICRUSt2 functional prediction indicated that disturbed pathways such as lipid metabolism and carbohydrate metabolism were notably reversed.The Qiqirigan-8 Tablets group showed superior improvements in all the above indices compared with the Qiqirigan-8 Pills group and the two negative control groups.Conclusion Qiqirigan-8 Tablets positively regulate intestinal microbiota in hyperlipidemic golden hamsters induced by a high-fat diet,concurrently exerting lipid-lowering and hepatoprotective effects,with the modified tablet for-mulation demonstrating superior efficacy.