基于"长江春2号×渝蜀鲜2号"F2群体的大豆叶绿素含量QTL动态分析OA

Soybean(Glycine max L.)is a major food crop in China and serves as an important source of edible oil and plant-based protein.Chlorophyll influences photosynthetic rate and is involved in biological processes such as soybean seed development.In order to advance the genetic dissection of chlorophyll content and facilitate marker-assisted breeding,an F2 population of 186 individuals derived from a cross between Changjiangchun 2(CJC2)and Yushuxian 2(YSX2)were used as the mapping population.Based on a genetic linkage map containing 675 molecular markers constructed in our laboratory,QTL analysis was performed using chlorophyll content data measured at three developmental stages in 2024,including the beginning flowering stage(R1),the beginning pod stage(R3),and the full seed stage(R6).A total of 32 chlorophyll QTLs were identified across the three stages,distributed across 18 linkage groups,of which four QTL intervals were consistently detected across two stages.According to functional annotations of the soybean reference genome,two genes related to chlorophyll metabolism were identified within these four intervals,namely Glyma.01G058600 and Glyma.15G215900.Among them,Glyma.15G215900 encodes a key enzyme in the chlorophyll degradation pathway,while Glyma.01G058600 is capable of binding chlorophyll a and carotenoid molecules,playing an important role in light energy harvesting and transfer.