连作与轮作对大豆和玉米根际土壤细菌微生态的影响OA
Influences of Continuous Cropping and Rotation on Rhizosphere Soil Bacterial Microecology of Soybean and Maize
为促进作物土壤优化并提高作物产品质量,采用传统微生物培养技术与Illumina MiSeq高通量测序技术相结合,研究不同种植制度下大豆、玉米根际土壤细菌的群落结构组成、丰度变化、菌群分布与土壤环境因子的相关性.结果表明,长期大豆连作、玉米连作、豆玉轮作种植模式下细菌群落多样性表现为大豆连作＞玉米连作＞豆玉轮作;细菌群落丰富度表现出大豆连作＞玉米连作＞豆玉轮作;大豆连作、玉米连作、豆玉轮作土壤细菌群落在门水平上菌群结构基本一致,优势菌门主要为酸杆菌门(Acidobacteriota 17.25％～32.14％)、变形菌门(Proteobacteria 19.25％～24.42％)、拟杆菌门(Bacteroidota 9.58％～18.73％),但豆玉轮作中变形菌门相对丰度(24.40％)、拟杆菌门相对丰度(18.76％)明显高于大豆连作相对丰度(19.41％)和玉米连作相对丰度(19.32％),而酸杆菌门相对丰度(17.27％)明显低于大豆连作相对丰度(29.09％)和玉米连作相对丰度(32.07％);土壤速效钾含量是影响优势菌群酸杆菌门、变形菌门、拟杆菌门群落分布的主要环境因子.综上,大豆连作会提高细菌多样性和丰富度,可通过调节速效钾含量改变微生物的群落结构.
In order to explore the influence of continuous cropping and rotation cropping systems on the diversity of the rhizosphere soil microbial community of soybean and maize,and to provide a reference basis for the optimization of crop soil and the improvement of crop product quality.The traditional microbial cultivation technology was combined with the Illumina MiSeq high-throughput sequencing technology.The research was carried out from the aspects of the composition of the community structure,the changes in abundance,and the correlation between the distribution of the bacterial community and soil environmental factors of the rhizosphere soil bacteria of crops under different cropping systems.Under the long-term continuous cropping of soybean,continuous cropping of maize,and soybean-maize rotation planting patterns,the diversity of the bacterial community was in the order of continuous cropping of soybean＞continuous cropping of maize＞soybean-maize rotation;the richness of the bacterial community showed continuous cropping of soybean＞continuous cropping of maize＞soybean-maize rotation.The structures of the bacterial communities in the soils of continuous cropping of soybean,continuous cropping of maize,and soybean-maize rotation at the phylum level were basically the same.The dominant bacterial groups were mainly Acidobacteriota(17.25％-32.14％),Proteobacteria(19.25％-24.42％),and Bacteroidota(9.58％-18.7 3％).However,the relative abundances of Proteobacteria(24.40％)and Bacteroidota(18.7 6％)in the soybean-maize rotation were significantly higher than those in the continuous cropping of soybean(19.41％)and continuous cropping of maize(19.32％),while the relative abundance of Acidobacteriota(17.27％)was significantly lower than that in the continuous cropping of soybean(29.09％)and continuous cropping of maize(32.07％).The content of available potassium(AK)in the soil was the main environmental factor affecting the community distribution of the dominant bacterial groups Acidobacteriota,Proteobacteria,and Bacteroidota.Continuous cropping of soybean can increase the diversity and richness of bacteria,and the community structure of microorganisms can be changed by regulating the content of available potassium(AK).
齐国超;王丽娜;姜泽宇;顾鑫;王迪
黑龙江省农业科学院大庆分院,黑龙江大庆 163316黑龙江省农业科学院大庆分院,黑龙江大庆 163316||国家盐碱地综合利用技术创新中心大庆试验站,黑龙江大庆 163316黑龙江省农业科学院大庆分院,黑龙江大庆 163316黑龙江省农业科学院大庆分院,黑龙江大庆 163316黑龙江省农业科学院大庆分院,黑龙江大庆 163316||国家盐碱地综合利用技术创新中心大庆试验站,黑龙江大庆 163316
高通量测序种植制度群落结构丰度环境因子
high-throughput sequencingplanting systemcommunity structureabundanceenvironmental factor
《黑龙江农业科学》 2026 (5)
1-8,8
黑龙江省省属科研院所科研业务费项目(CZKYF2024-1-C005)黑龙江省农业科技创新跨越工程农业关键技术重点攻关项目(CX23GG09).
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