雪上一枝蒿总生物碱及其三种生物碱类单体对MH7A细胞毒性及抗炎活性研究OA

Objective:To investigate the toxicity and in vitro anti-inflammatory activity of Aconitum brachypodum Diels total alkaloids and its three alkaloid monomers of on MH7A cells.Methods:MH7A cells were selected as experimental subjects,and the effects of total alkaloids of Aconitum brachypodum Diels alkaloids and its three alkaloid monomers on the cell viability of MH7A were determined by CCK-8 assay;The effect of total alkaloids of Aconitum brachypodum Diels alkaloids and its three al-kaloid monomers on the migration of MH7A cells was examined by the cell-scratch method;An in vitro rheumatoid arthritis(RA)cell model was established by tumor necrosis factor(TNF-α)stimulation of MH7A cells,and the expression of the inflam-matory factors prostaglandin E2(PGE-2),interleukin-6(IL-6),and interleukin-1β(IL-1β)in the supernatants of the cells was determined by ELISA.Results:The maximum cytotoxicity-free drug concentrations of each drug according to the CCK-8 assay were 1 140 μg/mL for Bullatine A,750 μg/mL for Bullatine B,100 μg/mL for total alkaloids of Aconitum brachypodum Diels,and 50 μg/mL for Aconitine officinale;The order of the inhibitory effects on the migration of MH7A cells in each dosing group was as follows:Aconitine>Bullatine B>total alkaloids of Aconitum brachypodum Diels>Bullatine A;Total alkaloids of Aco-nitum brachypodum Diels,Bullatine A,Bullatine B,and Aconitine inhibited the amount of PGE2,IL-6,and IL-1β production in MH7A cells after TNF-α induction.Conclusion:Total alkaloids of Aconitum brachypodum Diels,Bullatine A,Bullatine B,and Aconitine all inhibited the secretion of cytokines PGE2,IL-6,and IL-1β from MH7A cells in a concentration-dependent manner.