基于特征图谱和含量测定评价薄树芝与6种灵芝属药材多糖质量OA
Evaluation of Polysaccharide Quality Between Magoderna subresinosum(Murrill)Steyaert and Six Species of Ganoderma Medicinal Based on Characteristic Chromatograms and Content Determination
[目的]建立薄树芝单糖特征图谱并测定主要单糖含量,探讨薄树芝与其他6种灵芝属药材的差异.[方法]采用水提醇沉法提取样品粗多糖,盐酸水解产物通过1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化后进行高效液相色谱(HPLC)特征图谱分析,内标法测定D-甘露糖、D-葡萄糖及半乳糖的含量.[结果]23批薄树芝和24批其他6种灵芝属药材(赤芝、紫芝、无柄赤芝、平盖灵芝、树舌灵芝、黑灵芝)标定及指认了6个特征峰,分别为D-甘露糖、D-葡萄糖醛酸、D-葡萄糖、半乳糖、D-木糖、L-岩藻糖,其中D-葡萄糖为最强峰,D-甘露糖和半乳糖次之,D-木糖最弱.但半乳糖与D-葡萄糖的峰面积比值则有一定差异,其中,薄树芝的峰面积比值((x)±s)为0.126±0.046,基本低于紫芝、黑灵芝的比值,高于赤芝、无柄赤芝、平盖灵芝、树舌灵芝的比值.而薄树芝中D-甘露糖、D-葡萄糖、半乳糖含量及三者含量((x)±s)依次为0.134%±0.059%、0.972%±0.270%、0.113%±0.038%、1.225%±0.318%,平均总含量高低依次为树舌灵芝、薄树芝、紫芝、黑灵芝、赤芝、无病赤芝、平盖灵芝.[结论]HPLC柱前衍生化法测定D-甘露糖、D-葡萄糖、半乳糖,方法稳定可行,可评价薄树芝与其他6种灵芝属药材多糖质量差异.
Objective To establish characteristic chromatograms of monosaccharides derived from Magoderna subresinosum(Murrill)Steyaert and determine the contents of its main monosaccharides,thereby exploring the differences between M.subresinosum(Murrill)Steyaert and six other Ganoderma species.Methods Crude polysaccharides were extracted using water extraction and alcohol precipitation.The hydrochloric acid hydrolysates were derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP)and analyzed by high-performance liquid chromatography(HPLC)to generate characteristic chromatograms.The contents of D-mannose,D-glucose,and galactose were determined using the internal standard method.Results Six characteristic peaks were identified and annotated across 23 batches of M.subresinosum(Murrill)Steyaert and 24 batches of six other Ganoderma species[Ganoderma lucidum(Leyss.ex Fr.)Karst,G.sinense Zhao,Xu et Zhang,G.daipingshanense J.D.Zhao,G.applanatum(Pers.)Pat,G.applanatum(Pers.ex Gray)Pat,G.atrum].These peaks corresponded to D-mannose,D-glucuronic acid,D-glucose,galactose,D-xylose,and L-fucose.D-glucose exhibited the highest peak intensity,followed by D-mannose and galactose,while D-xylose showed the weakest intensity.However,the peak area ratio of galactose to D-glucose demonstrated notable variation.The peak area ratio for M.subresinosum(Murrill)Steyaert((x)±s)was 0.126±0.046,which was generally lower than that of G.sinense Zhao,Xu et Zhang and G.atrum but higher than that of G.lucidum(Leyss.ex Fr.),G.daipingshanense J.D.Zhao,G.applanatum(Pers.)Pat,G.applanatum(Pers.ex Gray)Pat.The mean contents((x)±s)of D-mannose,D-glucose,and galactose,along with their total content in G.capense,were 0.134%±0.059%,0.972%±0.270%,0.113%±0.038%,and 1.225%±0.318%,respectively.The average total monosaccharide content in descending order was G.applanatum(Pers.ex Gray)Pat,Ganoderma capense(Lioyd)Teng,M.subresinosum(Murrill)Steyaert,G.sinense Zhao,Xu et Zhang,G.atrum,G.lucidum(Leyss.ex Fr.)Karst,G.daipingshanense J.D.Zhao,G.applanatum(Pers.)Pat.Conclusion The pre-column derivatization HPLC method for determining D-mannose,D-glucose,and galactose is stable and feasible,and can be effectively used to evaluate the quality differences in polysaccharides between M.subresinosum(Murrill)Steyaert and six other Ganoderma species.
黄月纯;刁锦钧;刘桂源;梁芷韵;黄春蕾;丘振文
广州中医药大学第一附属医院,广东 广州 510405||广东省中医临床研究院,广东省岭南特色医院制剂转化工程技术研究中心,医疗机构中药制剂与中药新药转化广东省工程研究中心,广东 广州 510405广州中医药大学中药学院,广东 广州 510006广州华商学院健康医学院,广东 广州 511300广州中医药大学第一附属医院,广东 广州 510405广州中医药大学第一附属医院,广东 广州 510405广州中医药大学第一附属医院,广东 广州 510405||广东省中医临床研究院,广东省岭南特色医院制剂转化工程技术研究中心,医疗机构中药制剂与中药新药转化广东省工程研究中心,广东 广州 510405
医药卫生
薄树芝灵芝属多糖柱前衍生法高效液相色谱法特征图谱含量测定
Magoderna subresinosum(Murrill)SteyaertGanoderma speciespolysaccharidespre-column derivatizationhigh-performance liquid chromatography(HPLC)characteristic chromatogramcontent determination
《广州中医药大学学报》 2026 (6)
1661-1669,9
广州中医药大学第一附属医院国家中医药传承创新中心科研专项(青年基金)(编号:2023QN18)
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