基于DUS测试的贵州大豆遗传多样性分析OA

[Objective]The genetic diversity in basic traits of spring soybean varieties was analyzed based on DUS test to provide a reference for innovation and improvement of soybean germplasm resources in Guizhou.[Method]31 phenotypic traits of 19 spring soybean varieties from Guizhou were analyzed based on the DUS test standard for evaluating new plant varieties,and the diversity of traits was compared and comprehensively evaluated.[Result]There were 67 allelic variations with ranging from 1-5 among 31 basic traits of 19 spring soybean varieties.The average number of effective alleles was 1.58 ranging from 1.00-3.50.The average Shannon's diversity index was 0.46.The genetic diversity of quantitative traits was higher than that of qualitative traits and pseudo-quality traits,and the diversity level of different phenotypic traits was different.The median and mean values of 7 traits related to yield(plant height,bottom pod height,branch number,pod number,main stem node number,pod seed number and 100-grain weight)were close,and all of them had normality characteristics except for plant height.There were correlations between soybean basic traits mostly,and some traits were affected by multiple traits.The principal component analysis showed that the cumulative contribution rate of the top four principal components with eigenvalue>1 reached 80.783％.Among them,the contribution rate of 10 traits of hair density,and height,deciduousness,pod number,branch number,pod cracking,hypocotyl anthocyanin color intensity,maturity,legume seed number and seed coat cracking ratio of plant was higher.[Conclusion]There are differences in the diversity levels of different phenotypic traits in spring soybean varieties in Guizhou.The traits of hair density,and height,deciduousness,pod number,branch number,pod cracking of plant and so on can be used as the basis for identification of spring soybean germplasm resources in Guizhou.