不同种植模式高粱根际土壤细菌多样性及PICRUSt功能基因预测OA

[Objective]The differences in bacterial diversity and functional genes in sorghum rhizosphere soil under continuous cropping and crop rotation patterns were explored to clarify the regulatory effects of crop rotation on bacterial communities and their functions and provide a theoretical basis for alleviating continuous cropping obstacles and building a rational crop rotation system.[Method]The Hongyingzi(sorghum variety),Youyan 2020(rapeseed variety)and Yuzao(stem mustard variety)were used as the tested materials.The differences in bacterial community structure and functional genes of sorghum rhizosphere soil samples collected from sorghum continuous cropping pattern(CK),sorghum-rapeseed rotation pattern(YC)and sorghum-stem mustard rotation pattern(ZC)were determined by 16S rRNA gene V3-V4 region high-throughput sequencing combined with PICRUSt functional prediction technology.[Result]A total of 1 435 shared OTUs were identified among CK,YC,and ZC treatments,and accounted for 73.78％.YC treatment showed a higher relative abundance of Nitrospira,Rokubacteriales.Acidobacteria group and Proteobacteria group bacteriap25 As for 2C treatment,Chujiebacter,Intrasporangiaceae were not cultured,and the relative abundance of Lysobacter was lower.The heterogeneity of YC and ZC treatments with a tightly clustered community was lower than CK.There was no significant difference in functional genes at Class 1 level among CK,YC and ZC treatments,but significant differences in some functional genes at Class 2 and Class 3 level among CK,YC and ZC treatments.The functional genes related to antibiotic synthesis and amino acid biosynthesis enriched in YC treatment.The functional genes related to fatty acid metabolism and amino acid metabolism enriched in ZC treatment.The functional genes related to cytochrome xenobiotic metabolism enriched by P450 pathway in CK treatment.[Conclusion]The overlapping degree of bacterial community structure around sorghum rhizosphere soil under different cultivation patterns is higher.The crop rotation pattern can change soil bacterial communities and functional gene structure.The continuous cropping pattern produce a stress to soil environment.The soil environment under the sorghum-rapeseed rotation pattern may have a control potential against crop diseases.The soil environment under the sorghum-stem mustard rotation pattern may produce an oxidative stress.