孟河医派特色炮制酒洗当归对早期股骨头坏死大鼠股骨头微结构及相关信号通路的影响OA

Objective:To explore the influence of Menghe medical school's characteristic processed wine-washed angelica sinensis on femoral head microstructure,the levels of OPG,RANKL,RANK,Wnt1 and Wnt3A,and related signaling pathways in rats with early-stage osteonecrosis of the femoral head(ONFH).Methods:Eighteen rats with ONFH were randomly divided into three groups(n=six per group):the early-stage ONFH model group,the model+traditionally processed wine-baked Angelica sinensis group,and the model+Menghe medical school's characteristic processed wine-washed Angelica sinensis group.Rats in the early-stage ONFH model group were administered normal saline by gavage,those in the model+traditionally processed Angelica sinensis group were given traditionally processed Angelica sinensis(4 mg·kg-1)by gavage,and those in the model+Menghe medical school's characteristic processed wine-washed Angelica sinensis group were given Menghe medical school's characteristic processed wine-washed Angelica sinensis(4 mg·kg-1)by gavage.All groups received consecutive interventions for 30 days.Following the intervention,all rats were euthanized.Femoral bone thickness and bone mineral density(BMD)were evaluated by X-ray examination.Morphological changes in the femoral head bone tissue were observed using hematoxylin-eosin(HE)staining.The expression levels of OPG,Wnt1,Wnt3A,RANK,and RANKL in the femoral head bone tissue were detected by immunohistochemistry.Results:X-ray findings of the femurs showed that in the model group,there were multiple high-density sclerotic areas of various morphologies within the femur,along with increased bone thickness and a marked increase in BMD.In the model+traditional processed Angelica sinensis group and the model+Menghe medical school's wine-washed Angelica sinensis group,bone thickness and BMD were reduced.HE staining showed that the model group exhibited articular cartilage erosion,an uneven cartilage surface,absence of the superficial layer,and a disrupted tidemark.The model+traditional processed Angelica sinensis group showed articular cartilage damage,an irregular cartilage surface,and an incomplete tidemark,while the model+Menghe medical school's wine-washed Angelica sinensis group exhibited mild articular cartilage damage,a relatively smooth cartilage surface,and a relatively intact tidemark.Compared with the model group,both treatment groups(model+traditional processed Angelica sinensis and model+Menghe medical school's wine-washed Angelica sinensis)showed significantly increased protein expression levels of OPG,Wnt1,and Wnt3A,and significantly decreased protein expression levels of RANK and RANKL in the femoral head bone tissue(P<0.05).Furthermore,the improvement in these indicators was significantly better in the Menghe medical school's wine-washed Angelica sinensis group than in the traditional processed Angelica sinensis group(P<0.05).Conclusion:Menghe characteristic processed Angelica sinensis may treat ONFH by modulating the expression of proteins involved in the NF-κB,OPG/RANKL/RANK,and Wnt/β-catenin signaling pathways,thus suppressing inflammation and immune responses.