消肿止痛合剂对大鼠骨骼肌损伤后MyoD蛋白及mRNA表达的影响OA

Objective:To investigate the effect of Xiaozhong Zhitong Mixture on the repair and regeneration of injured skeletal muscle based on the expression of myogenic determination factor(MyoD)protein and mRNA in the skeletal muscle tissue of rat models after skeletal muscle injury.Methods:A total of 80 experimental rats were randomly divided into a blank group,a control group,a model group,and a treatment group using a random number table method,with 20 rats in each group.Rats in each group were further randomly subdivided into four subgroups according to the time points of day 1,day 7,day 14,and day 21,with 5 rats in each subgroup.The blank group received normal saline by gavage;the control group received ibuprofen solution by gavage;the model group received normal saline by gavage;and the treatment group received Xiaozhong Zhitong Mixture at a dose of 10 mL/kg by gavage.After the intervention,the expression level of MyoD mRNA was detected by RT-PCR,and the expression level of MyoD protein was detected by Western blotting.Results:Compared with the blank group,there was no significant difference in the expression levels of MyoD protein and mRNA in the skeletal muscle of the model group on day 1(P>0.05).However,on days 7,14,and 21,the expression levels of MyoD protein and mRNA in the skeletal muscle of the model group were gradually up-regulated over time(P<0.05).Compared with the model group,the expression levels of MyoD protein and mRNA in the skeletal muscle of the treatment group were significantly up-regulated on days 7 and 14(P<0.05).Conclusion:Xiaozhong Zhitong Mixture can promote the repair and regeneration of injured skeletal muscle by up-regulating the expression of MyoD protein and mRNA.