糖尿病肾病大鼠血清糖基化终末产物与肾小球足细胞裂孔膜蛋白Nephrin、Podocin的相关性研究OA

Objective:To investigate the correlation between serum glycosylation end products(AGEs)and the expression of glomerular podocyte lacunar membrane proteins Nephrin and Podocin in diabetic nephropathy(DN)rats to elucidate the mechanism of AGEs in DN podocyte injury.Methods:Twenty-four Wistar rats were randomly divided into a control group and an experimental group,and the experimental group was fed by high-fat and high-sugar chow combined with streptozotocin(STZ)to induce DN model.The levels of serum AGEs were detected by ELISA,and the expressions of Nephrin and Podocin in renal tissues were detected by Western blot and immunohistochemistry,and the relation-ship between AGEs and podocyte proteins was evaluated by Pearson correlation analysis.Results:The level of serum AGEs in the experimental group(356.82±42.57)ng/mL was significantly higher than that in the control group(128.35±21.64)ng/mL(P<0.01),and the expression of Nephrin and Podocin was significantly lower(P<0.01).Correlation analysis showed that serum AGEs levels were significantly negatively correlated with Nephrin(r=-0.655)and Podocin(r=-0.639)expression(P<0.01).Conclusion:Elevated levels of serum AGEs in DN rats were closely correlated with decreased expression of podocyte injury lacunar membrane proteins Nephrin and Podocin,suggesting that AGEs may be involved in the pathogenesis of DN by impairing the structural integrity of the podocyte injury,and providing a potential target for early intervention in DN.