基于牛血清蛋白修饰的超顺磁性氧化铁双模态结直肠癌分子探针的制备及验证OA

Objective To developed a cell-targeted superparamagnetic iron oxide fluorescence-magnetic resonance dual-signal molecular probe,Cy5.5-antiGlut1-BSA⋅SPIONs was prepared.To evaluate its basic properties and explore cell fluorescence and magnetic resonance imaging capabilities.Methods Oil-soluble SPIONs were synthesized by high-temperature hot injection;The water-soluble BSA⋅SPIONs probe precursor was synthesized by phacoemulsification;After coupling with the anti-tumor polyclonal antibody(Glut1)and the infrared fluorescein Cy5.5,the Cy5.5-antiGlut1-BSA SPIONs targeted dual-signal molecular probe was prepared.The key properties such as morphology,particle size,optical properties and relaxation rate of the Cy5.5-antiGlut1-BSA·SPIONs probe were basically characterized.Furthermore,the Cy5.5-antiGlut1-BSA·SPIONs molecular probe was innovatively used to observe the expression and distribution of Glut1 at the tumor site in tissue biopsy under a fluorescence confocal microscope.Results This diameter of this molecular probe was 20-40 nm and it exhibited colloidal stability and biocompatibility under both acidic and alkaline conditions.It exhibited strong fluorescence and high relaxation(r2:129.92 s-1 per mmol/L Fe3+).The T2 signal of magnetic resonance imaging gradually increased with the increase of probe concentration.Through cytotoxicity and histological toxicity tests,this molecular probe showed non-toxic.Conclusion The Cy5.5-antiGlut1-BSA·SPIONs molecular probe possessed excellent fluorescence-magnetic resonance dual-mode imaging capabilities and specific targeting ability for colorectal cancer cells HCT116.It was a promising molecular probe for in vivo MRI-fluorescent diagnosis and ex vivo biopsy analysis of colorectal cancer cells.