基于拉曼光谱鉴别三种冰片的特征图谱OA

Objective Due to the extremely high similarity among the three types of borneol(Borneolum,Borneolum syntheticum,and l-Borneolum),Raman spectroscopy is used to establish the characteristic spectra for the rapid analysis of these three types of borneol,enriching the research data on the chemical components of borneol and providing a basis for the quality control of borneol.Methods Macroscopic identification,thin-layer chromatography(TLC)identification,specific rotation measurement,gas chromatography(GC),and Raman spectroscopy were used to identify the three types of borneol.Results The macroscopic characteristics of Borneolum,Borneolum syntheticum,and l-Borneolum are similar,with slight differences in shape,size,and color;their thin-layer chromatography shows high similarity,while significant differences were observed in in optical rotation;gas chromatography can identify the three based on the calculation of component contents from the chromatograms,and each has a unique Raman spectral"fingerprint"pattern.Conclusion Raman spectroscopy can quickly and non-destructively identify Borneolum,Borneolum syntheticum,and l-Borneolum,which is consistent with the test results of other methods.This method can provide a reference basis for their quality control and rational application.