菊粉对斑鳜幼鱼生长性能、消化酶活性、代谢功能及非特异性免疫的影响OA

[Objective]The aim of this experiment was to investigate the effects of inulin on the growth per-formance,digestive enzyme activity,metabolic function and non-specific immunity of juvenile Siniperca scherzeri.[Methods]A total of 360 juvenile S.scherzeri with initial body mass(IBM)of(7.40±0.07)g were selected and randomly divided into 4 groups with 3 replicates per group and 30 fish per replicate.Using crushed expanded feed for mandarin fish as the experimental basal diet,four types of diets were prepared with inulin ad-dition levels of 0%(control group,L1),0.5%(L2),1.0%(L3),and 1.5%(L4),respectively.The experiment las-ted for 60 days.[Results]1)The optimal growth performance of juvenile S.scherzeri was observed with an in-ulin addition level of 0.5%.At this level,final body mass(FBM),weight gain rate(WGR),and specific growth rate(SGR)significantly increased(P<0.05),while feed conversion ratio(FCR)significantly decreased(P<0.05).2)The intestinal digestive enzyme activity of juvenile S.scherzeri was improved at a dietary inulin level of 0.5%.The intestinal amylase(AMS)activity was significantly increased compared with the control group(P<0.05),the intestinal lipase(LPS)and intestinal trypsin(TPS)activities were increased compared with the control group(P>0.05).3)Adding inulin to feed could promote the serum lipid metabolism in juvenile S.scherzeri.The setum triglyceride(TG)content significantly decreased at a dietary inulin level of 1.5%,the serum cholesterol(CHO)and low-density lipoprotein cholesterol(LDL-C)levels at a dietary inulin level of 0.5%were significantly reduced compared with control group,the serum high-density lipoprotein cholesterol(HDL-C)content at a dietary inulin level of 1.0%-1.5%was significantly increased compared with control group.4)Juvenile S.scherzeri showed the highest protein metabilism at a dietary inulin level of 0.5%.At this level,The alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activities significantly de-creased.5)Adding inulin to feed can promote the non-specific immunity in juvenile S.scherzeri.The serum malondialdehyde(MDA)content of L2,L3 and L4 were significantly lower than that of the control group(P<0.05).The serum alkaline phosphatase(ALP)and acid phosphatase(ACP)activities significantly increased at a dietary inulin level of 0.5%(P<0.05).At this level,the activity of superoxide dismutase(SOD)was also higher than that of control group(P>0.05).[Conclusions]Under the conditions of this experiment,adding ap-propriate amounts of inulin to feed can promote the growth,digestion,metabolism and immunity of juvenile Siniperca scherzeri,and 0.5%inulin supplementation has a better effect.