基于转录组学和代谢组学分析选育系F5代与非选育系马苏大麻哈鱼卵基因表达及代谢物水平的差异OA

[Objectives]The effects of growth trait selection on the genes expression and metabolites levels in the eggs of landlocked Oncorhynchus masou were investigated by transcriptomics and metabolomics.[Methods]Eggs were collected from 15 non-selected and 15 fifth-generation(F5)selected landlocked O.masou.Differences in gene expression and metabolites levels between the eggs of the two species were analyzed by combining transcriptomics and metabolomics.[Results]The results of the transcriptome analysis revealed a total of 10 880 differentially expressed genes(DEGs),primarily enriched in ribosomes,oxidative phosphoryla-tion,lysosomes,and FoxO signaling pathways.Among these,the selected line of landlocked O.masou exhib-ited upregulated expression of gonadotropin(Gn),gonadotropin-releasing hormone(GnRH),and follicle-stimu-lating hormone(FSH)genes associated with oogenesis promotion,while downregulated expression of gonado-tropin-inhibiting hormone(GnIH)genes related to oogenesis inhibition.The results of the metabolomics analys-is revealed 583 differentially expressed metabolites(DEMs).Among these,the levels of vitellin(Vn),alkaline phosphatase(AKP),malate dehydrogenase(MDH),α-tocopherol,docosahexaenoic acid(DHA),and eicos-apentaenoic acid(EPA)were higher in the selected group compared to the non-selected group.Conversely,the levels of vitamin B2,spermine,and adenosine were lower in the selected group compared to the non-selected group.The combined analysis revealed that phospholipid,lysine,glucose and fatty acid metabolic signaling pathways participate in ovarian development by enhancing lipid and fatty acid matabolism.[Conclusions]Con-tinuous selection had significant effects on gene expression and metabolite levels of landlocked O.masou eggs,and phospholipid,lysine,glucose and fatty acid metabolic signaling pathways played important roles in the de-velopment of ovarian and eggs.