不同酶菌混合添加对甘蔗梢青贮水牛体外瘤胃发酵参数及微生物组成的影响OA

The aim of this study was to investigate the effects of mixed addition of different enzymes and bacte-ria on in vitro rumen fermentation parameters and microbial composition sugarcane top silage in buffalo.The 5 different additives of Lactobacillus plantarum,Pediococcus pentosaceus,cellulase,xylanase and glucose oxi-dase were mixed into sugarcane top silage by a 5-factor and 3-level orthogonal design method,and six better sugarcane top silage(groups T1 to T6)were selected for the in vitro rumen fermentation experiment of buffa-lo.The results showed as follows:1)the gas production of T3 group was significantly higher than that of other groups except group T4(P＜0.05),the hydrogen production of groups T3 and T4 was significantly higher than that of other groups(P＜0.05),the acetic acid and total volatile fatty acids contents of groups T5 and T6 were significantly higher than those of other groups(P＜0.05),and the ammonia nitrogen content of group T6 was significantly lower than that of groups T1,T2 and T5(P＜0.05).2)The Firmicutes relative abundance of group T4 was significantly higher than that of other groups except group T5(P＜0.05),and the Bacteroidetes relative abundance of groups T1,T2 and T3 was significantly higher than that of other groups T4,T5 and T6(P＜0.05).The Succiniclasticum relative abundance of group T4 was significantly higher than that of other groups(P＜0.05),the Rikenellaceae_RC9_gut_group relative abundance of group T2 was significantly higher than that of other groups(P＜0.05),the Prevotella relative abundance of group T3 was significantly higher than that of other groups(P＜0.05),and the Ruminobacter relative abundance of groups T4 and T6 was signif-icantly higher than that of other groups(P＜0.05).3)The acetic acid content was extremely significantly posi-tively correlated with the Ruminobacter relative abundance(P＜0.01),and the ammonia nitrogen content was significantly positively correlated with Rikenellaceae_RC9_gut_group(P＜0.05).In conclusion,the mixed ad-dition of different enzymes and bacteria have a certain effect on in vitro rumen fermentation parameters and mi-crobial composition of sugarcane top silage,the rumen fermentation quality and fermentation characteristics of sugarcane top silage of group T3 are better.[Chinese Journal of Animal Nutrition,2026,38(5):3810-3822]