茵陈黄酮对伊拉肉兔生长性能、血清炎症指标及盲肠微生物KEGG通路的影响OA

This experiment aimed to investigate the effects of dietary capillarisin on growth performance,serum inflammatory indices and cecal microflora KEGG pathways of Ira meat rabbits.Two hundred and forty 35-day-old weaned Ira meat rabbits with similar body weight were randomly assigned to 4 groups with 60 replicates per group and 1 rabbit per replicate.The control group was fed a basal diet,and the experimental groups were fed the basal diets supplemented with 0.001%(test group Ⅰ),0.002%(test group Ⅱ),and 0.003%(test groupⅢ)capillarisin,respectively.The experiment consisted of a 7-day adaptation period followed by a 21-day for-mal trial period.The results showed as follows:1)compared with the control group,the feed to gain ratio of test group Ⅱ was significantly decreased(P＜0.05).2)Compared with the control group,the contents of in-terleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and nuclear factor-κB(NF-κB)in serum of test groupⅠ,test group Ⅱ and test group Ⅲ were significantly decreased(P＜0.05);furthermore,the contents of IL-6,TNF-α and NF-κB in serum of test group Ⅱ were significantly lower than those of test group Ⅰ and test group Ⅲ(P＜0.05).3)Compared with the control group,at the phylum level,the cecal TM7 relative abun-dance of test group Ⅱ was significantly decreased(P＜0.05),and the Cyanobacteria relative abundance was significantly increased(P＜0.05).The linear discriminant analysis effect size(LEfSe)method was used to an-alyze the differences of cecal microbial marker species,compared with the control group,the marker species with higher relative abundance of test group Ⅱ were Mogibacteriaceae,Ruminococcus,Thermotogae,Kosmo-toga,Thermotogales and Thermotogaceae;compared with the test group Ⅰ,the marker species with higher relative abundance of test group Ⅱ were Thermotogae,Thermotogales,Thermotogaceae,Kosmotoga and Os-cillospira;compared with the test group Ⅲ,the marker species with higher relative abundance of test group Ⅱwere chloroplast,Streptophyta,Faecalibacterium,Cucumis and mitochondria.4)Compared with the control group,the enrichment levels of SUCSYN-PWY and PWY-7347 pathways of test group Ⅱ were significantly up-regulated(P＜0.05);compared with the test group Ⅲ,the enrichment level of CHLOROPHYLL-SYN pathway of test group Ⅱ was significantly up-regulated(P＜0.05).In summary,dietary supplemented with 0.002%capillarisin can reduce the feed to gain ratio and serum proinflammatory factor contents of meat rab-bits,improve the relative abundance of beneficial bacteria in intestine,enrichment level of CHLOROPHYLL-SYN pathway and intestinal environment,and enhance the body immunity.[Chinese Journal of Animal Nutri-tion,2026,38(5):3608-3617]