A random fractional 13C labeling strategy for P.pastoris expressed eukaryotic membrane proteins for solid-state NMR studiesOA
A random fractional 13C labeling strategy for P.pastoris expressed eukaryotic membrane proteins for solid-state NMR studies
Meihui Sang;Zejun Fan;Qiongqiong Ren;Jing Liu;Shaokai Zhao;Shenlin Wang
State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,ChinaState Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,ChinaState Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,ChinaCollege of Chemistry and Chemical Engineering,Peking University,Beijing,100087,ChinaCollege of Chemistry and Chemical Engineering,Peking University,Beijing,100087,ChinaState Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,China
Solid-state NMRSparse 13C labelingEukaryotic membrane proteinsP.pastoris expression system
Solid-state NMRSparse 13C labelingEukaryotic membrane proteinsP.pastoris expression system
《磁共振快报（英文）》 2026 (2)
97-106,10
All of the NMR experiments were conducted at the Beijing NMR Center,the NMR Facility at the National Center for Protein Sciences at Peking University,the National Center for Protein Science(Shanghai),and the 800 MHz spectrometer at East China University of Science and Technology.The work was supported by the National Key R&D Program of China(2024YFA0917100),the National Natural Science Foundation of China(22274050),the Shanghai Science and Technology Commission(contract number:23J21900300,24HC2810700)and the Fundamental Research Funds for the Central Uni-versities.We are grateful for the assistance of Dr.Zhi-jun Liu of the National Center for Protein Science,Shanghai,on NMR data collection.We also thank the staff members of the Nuclear Magnetic Resonance System at the National Facility for Protein Science in Shanghai(NFPS),Shanghai Advanced Research Institute,Chinese Academy of Sciences,China for providing technical support and assistance in data collection and analysis.
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