槐杞黄颗粒抑制银屑病炎症与增殖的作用机制:网络药理学与动物实验联合研究OA

Objective:To investigate the intervention mechanism of Huaiqihuang Granules in psoriasis vulgaris based on network phar-macology and animal experiments.Methods:Network pharmacology was used to predict the intervention mechanism of Huaiqihuang Granules in psoriasis.Active ingredient targets were screened using the HERB database and Swiss Target Prediction database.Genes related to psoriasis vulgaris were obtained from the GeneCard and DisGeNET databases.A Huaiqihuang Granules component-target-psoriasis network was constructed using Cytoscape software,and a protein-protein interaction(PPI)network diagram was drawn using the STRING database to analyze the core targets of Huaiqihuang Granules in treating psoriasis vulgaris.Gene Ontology(GO)function-al annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using the DAVID database to predict enriched signaling pathways,and the results were visually presented.Animal experiments:Sixty KM male mice were randomly divided into a blank group,model group,Yinxie Capsule group(0.6 g/kg),Huaiqihuang high-dose group(8 g/kg),Huaiqihuang medium-dose group(4 g/kg),and Huaiqihuang low-dose group(2 g/kg),with 10 mice in each group.A psoriasis ani-mal model was established by applying 5％imiquimod cream to the shaved back skin of the mice.After one week of treatment,the Psoriasis Area and Severity Index(PASI)score of the mouse skin lesions was assessed,and the degree of ear swelling and ear swelling rate were recorded.Toluidine blue staining was used to observe the number of mast cells in the back skin lesion tissue of the mice.Ser-um levels of IL-17 and TNF-α were detected by ELISA,and the expression of VEGF and Ki67 in the skin lesions of mice in each group was detected by immunohistochemistry.Results:A total of 1,016 drug-active ingredient targets and 1,169 psoriasis-related targets were identified after retrieval and screening,with 190 common targets between the drug and the disease.Poria cocos,Lycium barbarum,and Polygonatum sibiricum in Huaiqihuang Granules had 49,179,and 133 overlapping genes with psoriasis,respectively.GO func-tional enrichment analysis of the core targets of Huaiqihuang Granules yielded 2,639 biological processes,85 cellular components,and 205 molecular functions.The core targets were mainly associated with biological processes such as the regulation of inflammatory re-sponse and the regulation of cell apoptosis and proliferation.Compared with the model group,the Huaiqihuang high-dose group showed varying degrees of reduction in PASI score,degree of ear swelling,ear swelling rate,number of mast cells in the back skin,serum lev-els of IL-17 and TNF-α,and expression levels of VEGF and Ki 67 in the skin(P＜0.01).Conclusion:Huaiqihuang Granules can exert a therapeutic effect on psoriasis by inhibiting the inflammatory response and regulating cell apoptosis and proliferation.