连翘异型花柱植株果实发育及成分代谢研究OA

Objective:According to the theory of Chinese medicinal material quality,genetic factors play a dominant role in shaping the"morphological traits"and"metabolic traits"of organisms.Based on the academic concept of"genetic dominance theory of Chi-nese medicinal material quality,"this study aims to elucidate the patterns of fruit development and compositional metabolism in hetero-stylous Forsythia suspensa plants,providing a theoretical basis for high-quality cultivation and breeding.Methods:The apparent traits(longitudinal and transverse diameters)and contents of protein,crude fat,and soluble sugar in fruits of heterostyly F.suspensa plants at different developmental stages were measured and subjected to correlation analysis.The contents of nine active components were sim-ultaneously determined by HPLC,and their dynamic changes were analyzed.Results:The development of fruits in heterostylous F.suspensa plants corresponds to the growth characteristics of"fast-slow-steady".Based on integrated analyses of external morphological changes and internal seed embryo development,the fruit development process of heterostylous F.suspensa plants was classified into three periods:the young fruit stage,the middle fruit stage,and the mature stage.The L-type fruits of F.suspensa plant exhibited su-perior trait indices,including longitudinal diameter,transverse diameter,and quality,compared to the S-type fruits of F.suspensa plant overall.Significant differences were observed in the accumulation patterns of soluble sugars,proteins,and crude fat contents be-tween the fruit development of heterostylous F.suspensa plants.HPLC results showed statistically significant differences in the contents of nine active components in the fruits of heterostylous F.suspensa plants across different developmental stages.The content of forsythi-aside E exhibited a trend of initial decrease followed by an increase,whereas chlorogenic acid,rutin,quercetin,and phillygenin showed decreasing trends.In contrast,forsythoside I,pinoresinol-β-D-glucoside,forsythiaside A,and phillyrin initially increased and then decreased.During the young and middle fruit stages,the contents of forsythiaside A and phillyrin in the fruits of L-type plants were substantially higher than those in S-type plants,with statistically significant differences(P＜0.01).In the mature fruit stage,the forsythiaside A content in S-type plants was significantly higher than that in L-type plants,also showing statistically significant differ-ence(P＜0.01).Conclusion:The developmental trends of fruits in heterostylous F.suspensa plants are generally consistent,but the physiological indicators and the patterns of active component contents vary due to floral heteromorphism.