乌头汤调控lncRNA MALAT1改善骨关节炎软骨细胞铁代谢紊乱机制研究OA

Objective:To investigate whether Wutou Decoction regulates interleukin-1β(IL-1β)-induced inflammatory iron metabolism disorder in chondrocytes through the regulatory role of lncRNA MALAT1.Methods:Chondrocytes were extracted from the bilateral knee joints of 20 male SPF-grade C57BL/6 mice for in vitro culture.An in vitro chondrocyte inflammation model was induced by treatment with 10 ng/mL IL-1β for 24 hours,and lncRNA MALAT1 overexpressing cells were constructed.The chondrocytes were divided into five groups:blank group,model group,Wutou Decoction group,lncRNA MALAT1 overexpression group and lncRNA MALAT1 overexpression+Wutou Decoction group.Real-time PCR was used to detect the relative expression level of lncRNA MALAT1 in chondrocytes of each group.Western blot was used to determine the protein expression levels of MMP13,GPX4,ACSL4,and COX2 in chondrocytes of each group.Results:Real-time PCR results showed that the expression of lncRNA MALAT1 was significantly increased in the IL-1β-induced chondrocyte inflammation model(P<0.05).Western blot results showed that,compared with the blank group,the model group showed an upward trend in the expression levels of MMP13,ACSL4 and COX2,while the expression level of GPX4 decreased(P<0.05).Compared with the lncRNA MALAT1 overexpression group,the lncRNA MALAT1 overexpression+Wutou Decoction group had decreased expression levels of MMP13,ACSL4,and COX2,and increased expression level of GPX4(P<0.05).Compared with the model group,the Wutou Decoction group showed decreased expression levels of MMP13,ACSL4,and COX2(P<0.05),and an increasing trend in GPX4 expression(P<0.05).Conclusion:Wutou Decoction can reduce the expressions of MMP13,ACSL4,and COX2,and enhance the protein expression level of GPX4 by regulating lncRNA MALAT1,thereby regulating the process of iron metabolism disorder in OA chondrocytes and delaying the progression of chondrocyte degeneration.