Recommendation for biological evaluations on biodegradable magnesium-based materials:Based on the coupling impact of pH value and Mg^(2+)on cells and bacteriaOA
In vitro biological evaluations are important in developing and clinically approving biodegradable magnesium(Mg)-based devices.However,such methods specified in ISO Standards for these in vitro evaluations are flawed.The particular concern is the excessive inhibition of Mg extracts on cells and bacteria,attributed to the increased pH value and Mg^(2+)concentration due to Mg degradation.To figure out this issue,the current investigation detailed the coupling impact of these two factors on the viability of normal cells,tumor cells and bacteria.The results showed that the response of cells and bacteria to Mg^(2+)heavily depended on the medium pH value.Normal human dermal fibroblasts(NHDF)and human non-small-cell lung cancer cells(H_(2)3)exhibited a high tolerance to Mg^(2+)under the neutral condition,while human umbilical vein endothelial cells(HUVEC)under the alkaline condition.The alkaline condition coupled with increased Mg^(2+)concentrations could produce antibacterial effects against Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli),while the tolerance of these two bacteria to Mg^(2+)at different pH values was also different.The overlap analysis on the survival predominant regions of these cells and bacteria indicated that Mg degradation could not achieve a win-win situation of antisepsis and the safety of the two normal cells by affecting the medium pH value and Mg^(2+)concentration.However,increasing Mg^(2+)concentration and pH might have anti-H_(2)3 effects without inducing cytotoxicity to NHDF and/or HUVEC cells.Based on these results,a recommendation to evaluate the biological effects of magnesium-based materials in terms of their interfacial characteristics during degradation and associated key environmental thresholds influencing the behavior of cells and bacteria was proposed.
Yue Zhang;Tingjie Nie;Yulong Wu;Zhiqiang Gao;Dongfang Chen;Kun Qian;Genzhi Jiang;Huan Liu;Yi Shao;Cheng Wang;Chenglin Chu;Feng Xue;Yuanding Huang;Norbert Hort;Jing Bai
Institute of Metallic Biomaterials,Helmholtz-Zentrum Hereon,Geesthacht 21502,Germany School of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,ChinaSchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,ChinaInstitute of Surface Science,Helmholtz-Zentrum Hereon,Geesthacht 21502,GermanySchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,ChinaSchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,ChinaSchool of Materials Science and Engineering,Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology,Nanjing Institute of Technology,Nanjing 211167,ChinaInstitute of Metallic Biomaterials,Helmholtz-Zentrum Hereon,Geesthacht 21502,GermanyCollege of Materials Science and Engineering,Hohai University,Nanjing 211100,ChinaSchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,ChinaSchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,ChinaSchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,ChinaSchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,ChinaInstitute of Metallic Biomaterials,Helmholtz-Zentrum Hereon,Geesthacht 21502,GermanyInstitute of Metallic Biomaterials,Helmholtz-Zentrum Hereon,Geesthacht 21502,Germany Institute of Product Technology and Systems,Leuphana University Lüneburg,Lüneburg 21335,GermanySchool of Materials Science and Engineering,Jiangsu Key Laboratory for Advanced Metallic Materials,Southeast University,Nanjing 211189,China Institute of Biomedical Devices(Suzhou),Southeast University,Suzhou 215163,China
医药卫生
In vitroViabilityCytotoxicityAntibacterial activityISO Standards
《Journal of Magnesium and Alloys》 2026 (5)
P.190-204,15
the China Scholarship Council for the award of fellowship and funding(Nos.202206095009,202106050030,202106890013)German Academic Exchange Service(DAAD)for the award of fellowship and funding(No.91870848)supported by the Science and Technology Project of Jiangsu Province(BE2023719)Suzhou Science and Technology Project(SJC2023005,SZS2023023)。
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