Advancing synthetic biology for sustainable one-carbon biomanufacturingOA
Synthetic biology presents a promising pathway for transforming abundant one-carbon(C1)compounds,such as CO_(2),CH4,and methanol,into value-added products using engineered microbes,providing a sustainable alternative to conventional biomanufacturing methods without competing with food resources.Despite the growing use of C1 feedstocks as supplementary carbon sources,their use as sole carbon substrates remains challenging because of inefficient enzyme kinetics,metabolic imbalances,and incompatibility between heterologous pathways and host physiology.This review presents recent progress in the engineering of natural and synthetic microbial chassis,focusing on the dual role of rational design and artificial intelligence(AI)in aligning exogenous C1-assimilating modules with endogenous metabolic networks.We propose that overcoming these bottlenecks requires system-level integration of multi-omics-guided strain optimization,AI-assisted protein engineering,and dynamic pathway regulation.By bridging foundational discoveries with scalable applications,this review provides a reference for the development of scalable and sustainable microbial platforms for the high-value conversion of C1 compounds,ultimately contributing to carbon neutrality and the mitigation of climate change.
Chunjun Zhan;Tong Liu;Yu Chen;YuDian Zhu;Xin Li;Jianming Liu;Qun Zhao;Xiulai Chen;Anping Zeng
Center of Synthetic Biology and Integrated Bioengineering,School of Engineering,Westlake University,Hangzhou 310024,China College of Engineering,Westlake University,Hangzhou 310030,China Key Laboratory of Intelligent Low-carbon Biosynthesis in Zhejiang Province,Hangzhou 310024,ChinaCenter of Synthetic Biology and Integrated Bioengineering,School of Engineering,Westlake University,Hangzhou 310024,China College of Engineering,Westlake University,Hangzhou 310030,China Key Laboratory of Intelligent Low-carbon Biosynthesis in Zhejiang Province,Hangzhou 310024,ChinaKey Laboratory of Quantitative Synthetic Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institute Technology,Chinese Academy of Sciences,Shenzhen 518055,ChinaCenter of Synthetic Biology and Integrated Bioengineering,School of Engineering,Westlake University,Hangzhou 310024,China College of Engineering,Westlake University,Hangzhou 310030,China Key Laboratory of Intelligent Low-carbon Biosynthesis in Zhejiang Province,Hangzhou 310024,ChinaCenter of Synthetic Biology and Integrated Bioengineering,School of Engineering,Westlake University,Hangzhou 310024,China College of Engineering,Westlake University,Hangzhou 310030,China Key Laboratory of Intelligent Low-carbon Biosynthesis in Zhejiang Province,Hangzhou 310024,ChinaCenter of Synthetic Biology and Integrated Bioengineering,School of Engineering,Westlake University,Hangzhou 310024,China College of Engineering,Westlake University,Hangzhou 310030,China Key Laboratory of Intelligent Low-carbon Biosynthesis in Zhejiang Province,Hangzhou 310024,ChinaSchool of Biotechnology,Jiangnan University,Wuxi 214122,ChinaSchool of Biotechnology,Jiangnan University,Wuxi 214122,ChinaCenter of Synthetic Biology and Integrated Bioengineering,School of Engineering,Westlake University,Hangzhou 310024,China College of Engineering,Westlake University,Hangzhou 310030,China Key Laboratory of Intelligent Low-carbon Biosynthesis in Zhejiang Province,Hangzhou 310024,China
生物科学
BiomanufactoryGreenhouse effectOne-carbon compoundsSynthetic biologyThird-generation biomanufacturing
《Green Carbon》 2026 (1)
P.43-62,20
financially supported by the National Key R&D Program of China(2023YFA0914000)the Zhejiang Key Laboratory of Low-Carbon Intelligent Synthetic Biology(2024ZY01025).
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