Stepwise engineering of Clostridium ljungdahlii for efficient ethanol production from single-carbon gasesOA
Ethanol,a cornerstone biofuel,has been successfully produced on an industrial scale via fermentation of industrial off-gases(CO/CO_(2))using gas-fermenting Clostridium strains.To further enhance clostridial ethanol synthesis during gas fermentation,efforts have focused on the rational design and genetic modification of Clostridium strains.However,progress remains limited,indicating substantial potential for further optimization and improvement.In this study,we systematically and genetically engineered Clostridium ljungdahlii DSM 13528,a well-characterized gas-fermenting Clostridium strain,to achieve highly efficient ethanol synthesis via gas fermentation.By implementing a three-pronged metabolic strategy—strengthening ethanol synthesis pathways,improving acetate assimilation,and reducing 2,3-butanediol byproduct formation—we maximized carbon flux toward ethanol biosynthesis.The optimized strain exhibited a remarkable ethanol synthesis capacity for fermenting a CO–CO_(2)–H_(2)mixture,producing 30.1 g/L of ethanol within 102 h.This study established fundamental metabolic engineering principles for developing high-performance gas-fermenting Clostridium strains for sustainable ethanol synthesis from CO/CO_(2)substrates.This strategy can be readily adapted to industrial gas-fermenting Clostridium strains to enable more economically viable ethanol production.
Fengjuan Yang;Wanqi Li;Wei Ye;Peng Hu;Yinhua Lu;Weihong Jiang;Yang Gu
School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China CAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China Shanghai GTLB Biotech Co.,Ltd.,1688 North Guoquan Road,Shanghai 200438,China College of Life Sciences,Shanghai Normal University,Shanghai 200234,ChinaCAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China University of Chinese Academy of Sciences,Beijing 100049,ChinaCAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,ChinaShanghai GTLB Biotech Co.,Ltd.,1688 North Guoquan Road,Shanghai 200438,ChinaCollege of Life Sciences,Shanghai Normal University,Shanghai 200234,ChinaCAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,ChinaSchool of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China
化学化工
Clostridium ljungdahliiEthanol productionGas fermentationStrain designStrain modification
《Green Carbon》 2026 (1)
P.89-95,7
supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDC0110300)Frontier Technology R&D Program of Jiangsu Province(Grant No.BF2024079)Science and Technology Commission of Shanghai Municipality(Grant No.24HC2820800).
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