Construction of Shewanella oneidensis chassis for unbalanced fermentation with glycerol by adaptive laboratory evolutionOA
Shewanella oneidensis MR-1 is an electrochemically active bacterium with high potential for use in unbalanced fermentation.However,its efficiency in bioelectricity production using cheap substrates such as glycerol is limited.In this study,the ES3–16 evolved strain of Shewanella oneidensis was developed by adaptive laboratory evolution and exhibited an excellent ability to generate bioelectricity from glycerol with an electricity production efficiency of 502.1±13.9 mW/m^(2).The evolved strain with an acetoin-producing vector co-produced 473.3±21.2 mg/L acetoin and generated a power density of 1269.6±19.7 mW/m^(2) by unbalanced fermentation.Mutational analysis showed that increased glycerol utilization and altered cell morphology are vital for improving the bioelectricity synthesis capacity of S.oneidensis.Analysis of the genetic basis of the evolved strains provides important guidance for the efficient utilization of glycerol and unbalanced fermentation.
Xi Sun;Shiqi Luo;Shutian Kong;Guosheng Xin;Tao Chen;Feng Li;Hao Song;Zhiwen Wang
School of Life Sciences,Ningxia University,Yinchuan 750021,China Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Yinchuan 750021,ChinaState Key Laboratory of Synthetic Biology,Tianjin University,Tianjin 300072,China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,ChinaState Key Laboratory of Synthetic Biology,Tianjin University,Tianjin 300072,China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,ChinaSchool of Life Sciences,Ningxia University,Yinchuan 750021,China Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Yinchuan 750021,ChinaState Key Laboratory of Synthetic Biology,Tianjin University,Tianjin 300072,China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,ChinaState Key Laboratory of Synthetic Biology,Tianjin University,Tianjin 300072,China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,ChinaState Key Laboratory of Synthetic Biology,Tianjin University,Tianjin 300072,China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,ChinaSchool of Life Sciences,Ningxia University,Yinchuan 750021,China Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Yinchuan 750021,China State Key Laboratory of Synthetic Biology,Tianjin University,Tianjin 300072,China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China
化学化工
AcetoinAdaptive laboratory evolutionGlycerolShewanella oneidensisUnbalanced fermentation
《Green Carbon》 2026 (1)
P.96-103,8
financially supported by the National Natural Science Foundation of China(Grant Nos.NSFC-22278312 and 22478293)the Key Research and Development Program of Ningxia Hui Autonomous Region(Grant No.2024BEE02005)the Central Guidance for Local Science and Technology Development Projects(Grant No.2024FRD05057)the Natural Science Foundation of Tianjin(Grant No.23JCYBJC00670).
评论