Exploring the mechanism of Clerodendrum bungei Steud.alleviating bone cancer pain based on PI3K/AKT/NF-κB signalling pathwayOA
Background:Clerodendrum bungei Steud.(CBS)is a member of the genus Clerodendrum within the Lamiaceae family.Modern ethnobotanical investigations and phytochemical studies have demonstrated that CBS is rich in multiple bioactive constituents,including flavonoids and phenolic acids,which exhibit potential anti-inflammatory and analgesic properties.Bone cancer pain(BCP)represents one of the most refractory complications in patients with advanced malignancies,for which current analgesic therapies remain limited in efficacy and are frequently associated with significant adverse effects.Based on the traditional medicinal background of CBS and its multi-component characteristics,the present study integrates network pharmacology,molecular docking,and in vivo experimental validation to systematically elucidate the potential therapeutic targets and underlying molecular mechanisms by which CBS alleviates BCP.Methods:Rats were randomly divided into control,model,tramadol,and CBS groups,and a tibial BCP model was established using Walker 256 cells.Therapeutic effects were evaluated by body weight,paw withdrawal latency(PWL),imaging,and histopathology.Active compounds and targets of CBS were identified via network pharmacology,molecular docking,and molecular dynamics,and key signaling pathways were validated by western blotting and immunofluorescence.Results:In vivo experiments showed that CBS significantly increased the pain threshold and improved quality of life in rats with BCP(P<0.05).Network pharmacology identified 298 shared targets between CBS and BCP,mainly related to inflammation and neurosensitization,with the PI3KAKT pathway as a key mechanism.Molecular docking and dynamics revealed stable binding between naringenin and PIK3CD.Western blot and immunofluorescence further confirmed that CBS inhibited PI3K-AKT/NF-κB signaling and reduced inflammatory mediator release(P<0.05).Conclusion:This study explores the potential active compounds and molecular mechanisms underlying the efficacy of stinking peony,providing chemical bioinformatics insights for research and clinical applications in treating BCP.
Kang-Min Zhou;Chang-Pu Du;Jian Li;Wen-Qi Huang;Hua-Zheng Sun;Ai-Ling Liang;Dong-Xin Tang;Bing Yang;Feng-Xi Long
School of Basic Traditional Chinese Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China Tumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaTumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaTumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaTumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaTumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaTumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaTumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaTumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,ChinaSchool of Basic Traditional Chinese Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China Tumor Team,First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China
医药卫生
bone cancer painClerodendrum bungei Steud.PI3K/AKT/NF-κB signaling pathwayinflammatory cytokines
《Traditional Medicine Research》 2026 (9)
P.31-40,10
supported by the Guizhou Provincial Higher Education Center for Translational Medicine Research on Traditional Chinese and Ethnic Medicine in Cancer Prevention and Treatment(Grant No.Qianjiaojiaoji[2023]037)and the National Natural Science Foundation of China(82460959).
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