人参皂苷-莪术醇配伍影响线粒体-溶酶体互作调控卵巢癌细胞自噬机制的仿真研究OA

Objective Based on the traditional Chinese medicine theory of"Yang Transforms Qi,Yin Constitutes Form"and incorporating modern research on organelle interactions in autophagy regulation,this study aimed to computationally investigate the mechanism by which ginsenoside-curcumol combination regulates autophagy in ovarian cancer cells via influencing mitochondria-lysosome interactions.Methods A multi-agent model called"Traditional Chinese Medicine Intervention-Organelle Interaction-Ovarian Cancer Cell Autophagy"(TCMI-OI-OCCA)was constructed and simulated using NetLogo software to explore the system dynamics.Behavioral space experiments were conducted to obtain system behavior data under different parameter combinations.A random forest regression model was employed to analyze the importance of key variables on the system equilibrium.Results The random forest regression model demonstrated high goodness-of-fit(R² ≈ 0.999).Variable importance analysis revealed that cancer cell proliferation probability(cellp),simulation time(time),and autophagy probability(cella)were the most critical predictors influencing system equilibrium.The intervention parameters for ginsenoside and curcumol(gin,gine,cur,cure)also showed stable importance,indicating their definite role in modulating the system's dynamic balance.The mitochondria-lysosome positive and negative interaction parameters(pi,ni)did not contribute significantly to prediction accuracy in the current model.Conclusion The ginsenoside-curcumol combination may regulate the dynamic balance between autophagy and proliferation in ovarian cancer cells by modulating the mitochondria-lysosome interaction network.This effect embodies how the"Warming Yang and Resolving Stasis"therapeutic principle systemically regulates the balance of"Yang Transforms Qi,Yin Constitutes Form".