MC4R-rs6567160位点基因多态性与非酒精性脂肪性肝病患者纤维化程度的相关性OA

Objective:To investigate the association between MC4R-rs6567160 polymorphism and non-alcoholic fatty liver disease(NAFLD).Methods:A total of 2,032 NAFLD patients admitted to Chengdu Fifth People's Hospital were enrolled and stratified into no,mild,and moderate-to-severe fibrosis subgroups,alongside 1,920 healthy controls.Clinical and laboratory parameters were collected,and genotyping was performed via PCR.Group differences were compared to analyze the correlation between MC4R polymorphisms and the severity of liver fibrosis in NAFLD.Results:The frequency of the CC genotype was significantly higher in the NAFLD group compared to the control group(P<0.05).Patients with the CC genotype exhibited significantly higher levels of liver function markers,fibrosis indices,and liver stiffness measurements compared to those with TC and TT genotypes(P<0.05),with TC being intermediate.The proportion of the CC genotype increased with the severity of liver fibrosis,and corresponding clinical markers deteriorated accordingly(P<0.05).Alanine aminotransferase,aspartate aminotransferase,gamma-glutamyl transferase,hyaluronic acid,procollagen type Ⅲ,type Ⅳ collagen,laminin,liver stiffness measurement,controlled attenuation parameter,aspartate aminotransferase to platelet ratio index,fibrosis-4 index,and MC4R-rs6567160(CC genotype)were identified as independent risk factors for liver fibrosis severity in NAFLD patients(all P<0.05).ROC analysis demonstrated that the CC genotype had AUC values of 0.862 and 0.963(both P<0.001)in predicting the progression from no fibrosis to mild fibrosis and from mild to advanced fibrosis,respectively.Conclusion:The MC4R-rs6567160 polymorphism is significantly associated with NAFLD,and patients carrying the CC genotype are at a higher risk of liver fibrosis.These findings highlight the potential of the CC genotype as a biomarker for early diagnosis and risk stratification in NAFLD management.