扶正祛瘀方通过抑制NLRP3炎性小体激活治疗慢性子宫内膜炎的实验研究OA

Objective:To explore the therapeutic effect and potential mechanism of the Fuzheng Quyu for-mula on chronic endometritis(CE),and to verify whether it inhibits the activation of the NOD-like receptor thermal protein domain associated protein 3(NLRP3)inflammasome by eliminating reactive oxygen species(ROS).Methods:The CE rat model was constructed by intracavitary injection of phenol gelatin.24 Wistar rats were randomly divided into the control group,the model group,the doxycycline group(18 mg/kg/d),and the Fuzheng Quyu formula group(4.4g/kg/d).After the treatment intervention,the pathological changes of the endometrium were observed through hematoxylin and eosin(HE)staining;the levels of inflammatory factors in serum were detected by enzyme-linked immunosorbent assay(ELISA);the content of ROS in the endo-metrium was detected by immunofluorescence;and the expressions of NLRP3 inflammasome-related proteins[NLRP3,apoptosis-associated speck-like protein containing a CARD(ASC),caspase-1]were analyzed by Western blot.Results:Compared with the model group,the Fuzheng Quyu formula significantly improved pathological damages such as endometrial edema and inflammatory cell infiltration,reduced serum levels of interleukin(IL)-1β,IL-18,and tumor necrosis factor-α(TNF-α),decreased endometrial ROS accumulation,and inhibited the expression of NLRP3,ASC,and caspase-1 proteins.The therapeutic effect was comparable to that of doxycycline.Conclusion:The Fuzheng Quyu formula can exert therapeutic effects on CE by inhibit-ing the activation of NLRP3 inflammasome and reducing oxidative stress and inflammatory responses.