远缘杂交培育双单倍体丝瓜及其鉴定OA

In this study,we developed diploid Luffa through distant hybridization induction technology,providing a theoretical basis for the cultivation and genetic improvement of excellent Luffa varieties.Luffa hybrid variety TL 01(2n=26)was utilized as the female parent,five different male parents of Cucurbitaceae were hybridized.The obtained hybrid offspring were subjected to ploidy identification and selfing to obtain the first generation.Simple sequence repeats(SSR)markers were used to identify whether they were diploids.The results showed that a total of 12 plump seeds were harvested from the five hybrid combinations.However,only watermelon pollen-induced harvested seeds were successfully regenerated from two plants,labeled T-1 and T-2.63 seeds were harvested from the selfing of T-1 plants.Using SSR markers to identify the first generation of selfing,it was found that six pairs of SSR markers had consistent band patterns among the first generation of selfing,indicating that the regenerated plant was diploid.The fertility of diploid Luffa has been restored and can be propagated using seeds.