浙樱粉1号高效制种及纯度鉴定技术OA

Zheyingfen 1 is a high-quality,parthenocarpy cherry tomato variety selected by Zhejiang Academy of Agricultural Sciences and widely cultivated in Zhejiang Province.The low seed production yield and mechanical mixing during the seed production process of Zheyingfen 1 have hindered the further promotion and application of this variety.Therefore,researching efficient seed production and purity identification technology for this variety,reducing seed production costs,and improving seed purity,is of great significance for further expanding the nationwide influence of independently selected tomato varieties of Zhejiang Province.This study systematically investigated the effects of different pruning methods(single plant double stem,double plant single stem,double plant double stem)and different fertilization treatments(T1,application of balanced water-soluble fertilizer;T2,partial replacement of balanced water-soluble fertilizer with high-potassium water-soluble fertilizer on the basis of T1 treatment;T3,additional application of calcium fertilizer on the basis of T2 treatment)on the seed production yield and related agronomic traits of Zheyingfen 1.Meanwhile,based on single nucleotide polymorphism(SNP)data from resequencing,SNP molecular markers based on kompetitive allele specific PCR(KASP)were developed to establish a high-throughput and low-cost rapid identification technology for seed purity of Zheyingfen 1.The results showed that the seed yield of the single plant double stem combined with T3 fertilization treatment was significantly(p＜0.05)higher than that of other treatments,with a seed yield of 11.76 kg per 667 m2.In addition,the three KASP-SNP molecular markers developed in this study can accurately identify the genetic purity of the hybrid Zheyingfen 1.