杜仲叶总黄酮提取工艺优化及其抗氧化活性评价OA
Optimization of extraction process for total flavonoids from Eucommiae folium leaves and evaluation of their anti-oxidant activity
目的 优化杜仲叶总黄酮提取工艺,并评价其抗氧化活性.方法 在单因素试验基础上,以乙醇体积分数、液料比、浸泡时间、提取时间为影响因素,总黄酮、芦丁、异槲皮苷、紫云英苷、根皮苷、槲皮素、山柰酚含量及浸膏得率的综合评分为评价指标,AHP-熵权 TOPSIS 法结合 Box-Behnken 响应面法优化提取工艺.再测定 DPPH·、ABTS+·自由基清除率.结果 最佳条件为乙醇体积分数 60%,液料比 21∶1,浸泡时间 1.5 h,提取时间 2.0 h,提取次数 2次,各成分含量及浸膏得率分别为 79.671、0.131、1.127、0.035、2.143、0.855、0.157 mg/g 及 37.534%.总黄酮对 DPPH·、ABTS+·自由基的 IC50值分别为0.028、0.053 mg/mL.结论 该方法稳定可靠,可用于提取抗氧化活性较强的杜仲叶总黄酮.
AIM To optimize the extraction process for total flavonoids from Eucommiae folium Oliv.leaves,and to evaluate their anti-oxidant activity.METHODS With ethanol concentration,liquid-solid ratio,soaking time and extraction time as influencing factors,the comprehensive score for total flavonoids,rutin,isoquercitrin,astragalin,phlorizin,quercetin,kaempferol contents and extract yield as evaluation index,the anti-oxidant activity was optimized by AHP-entropy weight TOPSIS method combined with Box-Behnken response surface method.Subsequently,the scavenging rates of DPPH·,ABTS+·free radicals were determined.RESULTS The optimal conditions were determined to be 60%for ethanol concentration,21∶1 for liquid-solid ratio,1.5 h for soaking time,2.0 h for extraction time,and two times for extraction frequency,the various constituent contents and extract yield were 79.671,0.131,1.127,0.035,2.143,0.855,0.157 mg/g and 37.534%.The IC50 values of total flavonoids on DPPH·,ABTS+·free radicals were 0.028,0.053 mg/mL,respectively.CONCLUSION This stable and reliable method can be used for the extraction of total flavonoids from E.folium leaves with strong anti-oxidant activity.
董小丽;谢兴文;张家旭;李婉玉;马趣环;石晓峰
甘肃中医药大学,甘肃 兰州 730000||甘肃省医学科学研究院,甘肃 兰州 730050甘肃中医药大学附属医院,甘肃 兰州 730000甘肃中医药大学,甘肃 兰州 730000||甘肃省医学科学研究院,甘肃 兰州 730050甘肃中医药大学,甘肃 兰州 730000||甘肃省医学科学研究院,甘肃 兰州 730050甘肃省医学科学研究院,甘肃 兰州 730050甘肃省医学科学研究院,甘肃 兰州 730050
医药卫生
杜仲叶总黄酮提取工艺AHP-熵权TOPSIS法Box-Behnken响应面法抗氧化活性
Eucommiae folium Oliv.leavestotal flavonoidsextraction processAHP-entropy weight TOPSIS methodBox-Behnken response surface methodanti-oxidant activity
《中成药》 2026 (4)
1106-1114,9
甘肃省科技重大专项(22ZD6FA021-4)第七届兰州市人才创新创业大赛项目(2024-02-07)甘肃中医药大学研究生"创新创业基金"(2025CXCY-0-038)
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