基于HPLC指纹图谱、化学模式识别和含量测定评价黄花倒水莲质量OA

AIM To evaluate the quality of Polygala fallax Hemsl.METHODS The HPLC fingerprints were established,the analysis was performed on a 35℃thermostatic Waters CORTECS T3 C18 column(2.1 mm×150 mm,1.6 μm),with the mobile phase comprising of acetonitrile-0.1%formic acid flowing at 0.3 mL/min in a gradient elution manner,and the detection wavelength was set at 256 nm.Subsequently,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed,after which lancerin was used as an internal standard to calculate the relative correction factors of 7-O-methylmangiferin and polygalaxanthone Ⅲ,and the content determination was made by quantitative analysis of multi-components by single-marker(QAMS)method.RESULTS There were 5 common peaks in the fingerprints for 29 batches of medicinal materials with the similarities of more than 0.80(except for S2).Various batches of medicinal materials were clustered into two categories,7-O-methylmangiferin was quality differential marker.Three constituents showed good linear relationships within their own ranges(R2≥0.999 0),whose average recoveries were 95.34%-104.64%with the RSDs of 1.31%-2.30%.CONCLUSION This accurate and reliable method can provide evidence for the quality control of P.fallax.