基于HPLC指纹图谱、化学计量学和含量测定评价苗药热淋清颗粒质量OA

AIM To evaluate the quality of Miao medicine Relinqing Granules.METHODS The HPLC fingerprints were established,the analysis was performed on a 30℃thermostatic ShimNex CS C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of 0.2%phosphoric acid-methanol flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 254 nm.Subsequently,cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were performed,after which the contents of gallic acid,ellagic acid and quercitrin were determined.RESULTS There were 13 common peaks in the fingerprints for 47 batches of samples with the similarities of more than 0.871.Various batches of samples were clustered into two categories,three principal components demonstrated the accumulative variance contribution rate of 96.632%,and eight differential components were screened.Three constituents showed good linear relationships within their own ranges(R2≥0.999 7),whose average recoveries were 96.57%-101.85%with the RSDs of 0.70%-1.12%.CONCLUSION This simple,reliable and reproducible method can provide a scientific basis for the quality control and standard enhancement of Relinqing Granules.