食管上皮内瘤变与食管鳞状细胞癌真菌微生物特征分析OA
Fungal characteristics of esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma
目的 探索食管上皮内瘤变(esophageal intraepithelial neoplasia,EIN)及食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)食管真菌微生态的变化特征.方法 纳入 2023 年 2 月—2023 年 11 月就诊于河北医科大学第二医院行上消化道内镜检查的ESCC患者8例,EIN 患者5例,食管无病变者(normal control,NC)10例.将各组食管细胞刷样本进行内转录间隔区(internal transcribed spacer,ITS)测序,利用生物信息学方法进行深入分析.结果 三组间食管的真菌组成上存在差异,与 NC 组相比,EIN 组与 ESCC 组的食管真菌微生态多样性显著降低(P<0.05),而EIN 与 ESCC 组之间差异无统计学意义.相较于 NC 组,EIN 与 ESCC 两组的耶氏酵母属(Yarrowia)丰度均显著降低(P<0.05).相较于 EIN 组中金孢菌属(Chrysosporium)的相对丰度升高,ESCC 组中则呈现曲霉属(Aspergillus)的富集趋势.受试者工作特征(receiver operating characteristic,ROC)分析进一步提示,金孢菌属和曲霉属对 EIN 与 ESCC 具有一定的区分潜力,其曲线下面积(area under the ROC curve,AUC)分别为 70.0％(95％CI:38.2％～100.0％)和 73.8％(95％CI:49.5％～98.0％).结论 食管上皮内瘤变及食管鳞状细胞癌患者的真菌微生态组成发生改变,念珠菌属(Candida)、耶氏酵母属(Yarrowia)等真菌菌属可能与食管癌变过程相关.金孢菌属和曲霉属在本研究中呈现出与不同病变阶段相关的分布特征,有望为未来食管鳞状细胞癌的早期筛查和治疗提供微生物学证据.
Objective To explore the alterations in esophageal fungal microecology during the development and progression of esophageal intraepithelial neoplasia(EIN)and esophageal squamous cell carcinoma(ESCC).Methods A case-control study was designed,including 8 patients with ESCC,5 patients with esophageal intraepithelial neoplasia(EIN),and 10 Individuals with a normal esophagus(NC)who underwent upper gastrointestinal endoscopy at the Second Hospital of Hebei Medical University from February 2023 to September 2023.Esophageal cell brush samples from each group were sequenced for the internal transcriptional spacer(ITS)and analyzed using bioinformatics method.Results Significant differences in esophageal fungal composition were observed among the three groups.Compared with NC,the microecological diversity of esophageal fungi was significantly reduced in both the EIN and ESCC groups,while the diversity between EIN and ESCC groups was similar,with no statistically significant difference.Inter-group comparisons revealed that compared to the NC group,the abundance of Yarrowia in the EIN and ESCC groups was significantly reduced in both disease groups(P<0.05).While the relative abundance of Chrysosporium was higher in the EIN group,the ESCC group exhibited an enrichment trend of Aspergillus.Despite the limited sample size,ROC analysis suggested show certain potential of Chrysosporium and Aspergillus in differentiating EIN from ESCC groups.The area under the ROC curve(AUC)was 70.0％(95％CI:38.2％-100.0％)for Chrysosporium and 73.8％(95％CI:49.5％-98.0％)for Aspergillus.Conclusion The composition of esophageal fungi is altered in patients with esophageal intraepithelial neoplasia(EIN)and esophageal squamous cell carcinoma(ESCC).Fungal genera such as Candida and Yarrowia may be involved in the carcinogenic process.Chrysosporium and Aspergillus exhibited distribution patterns associated with different disease stages in this study.These findings provide exploratory microecological evidence that may inform future early detection and treatment strategies for ESCC.
宋怡康;郑琳;叶子渝;刘爱欣;马俊骥
河北医科大学第二医院消化内科 河北省消化病重点实验室 河北省消化病研究所 河北省消化系统疾病临床医学研究中心,石家庄 050000河北医科大学第二医院消化内科 河北省消化病重点实验室 河北省消化病研究所 河北省消化系统疾病临床医学研究中心,石家庄 050000河北医科大学第二医院消化内科 河北省消化病重点实验室 河北省消化病研究所 河北省消化系统疾病临床医学研究中心,石家庄 050000河北医科大学第二医院消化内科 河北省消化病重点实验室 河北省消化病研究所 河北省消化系统疾病临床医学研究中心,石家庄 050000河北医科大学第二医院消化内科 河北省消化病重点实验室 河北省消化病研究所 河北省消化系统疾病临床医学研究中心,石家庄 050000
食管鳞状细胞癌食管上皮内瘤变真菌微生态生物标志物
esophageal squamous cell carcinomaesophageal intraepithelial neoplasiafungal microecologybiomarker
《中国真菌学杂志》 2026 (2)
167-173,7
河北省2023年度医学科学研究课题(20230597)
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