11例西弗射盾子囊霉致耳道真菌感染的鉴定与药敏分析OA

Objective To analyze the antifungal susceptibility and resistance characteristics of Stephanoascus ciferrii(S.ciferrii)isolated from patients with otitis externa,and to offer evidence for the diagnosis and treatment of infections caused by rare fungi.Methods Among 280 specimens submitted to Sichuan Taikang Hospital from September 1,2023 to September 30,2025,eleven S.ciferrii strains were isolated from ear discharge samples of patients with otitis externa.The isolates were cultured and identified by MALDI-TOF MS and ITS sequencing,and antifungal susceptibility testing(AFST)was performed.Results All isolates could grow on blood agar and Sabouraud dextrose agar.On Candida CHROMagar,colonies appeared blue and firm.Microscopy revealed oval conidia arranged along the hyphae.MALDI-TOF MS identification was consistent with ITS sequencing(99％sequence identity).The isolates had high MICs to fluconazole(>32 µg/mL),whereas and relatively lower MICs to amphotericin B.Conclusion S.ciferrii is a rare opportunistic pathogen that can cause ear canal infections.Combining conventional culture with molecular identification improves diagnostic accuracy and may assist in guiding rational antifungal therapy.