亚麻抗倒伏性状的关联位点鉴定及候选基因分析OA

To explore the key loci and genes associated with flax lodging resistance,10 traits related to lodging resistance in 269 flax germplasm resources were identified and their genome-wide association analyses were con-ducted in 5 environments.The results showed that there were extensive phenotypic variations in flax lodging resis-tance traits,with the coefficient of variation of fresh weight being the largest(54.94％)and that of plant height being the smallest(13.68％).The phenotypic coefficient of variation of the 10 traits of flax lodging resistance is sorted in the following order:fresh weight＞stem breaking force＞stem bending resistance＞lignin＞cellulose＞puncture strength＞compressive strength＞center of gravity height＞stem thickness＞plant height.Under the five environ-ments,the generalized heritability of lignin in this group was the largest,reaching 65.72％,and the generalized heri-tability of the center of gravity height was the smallest,at 46.12％.The order of generalized heritability was lignin＞cellulose＞fresh weight＞stem breaking force＞compressive strength＞puncture strength＞stem bending force＞stem thickness＞plant height＞center of gravity height.Genome-wide association analysis of flax lodging resis-tance traits yielded 61 significantly associated SNP loci,with phenotypic interpretation rates ranging from 6.45％to 12.35％.A total of 72 candidate genes were annotated within a range of 10kb upstream and downstream of the signif-icantly associated SNP loci(-log10≥5.1),among which 4 genes were jointly detected in 2 environments.The candi-date gene LUSG00009364 for cell wall synthesis was detected at 1815 bp downstream of the SNP(group8:26914041)locus on chromosome 8,with a phenotypic interpretation rate of 10.89％.The DHHC palmitoyltransfer-ase gene LUSG00009997 detected at 6843 bp upstream of the SNP(group3:153196)locus on chromosome 3 has a phenotypic interpretation rate of 12.35％.The Ppx/GppA phosphatase gene LUSG00003108 was detected at 6043 bp upstream of the SNP(Group 11:23793243)locus on chromosome 11,and the pantothenic acid phosphorylase gene was detected at 6189 bp downstream of the SNP(Group 12:11454172)locus on chromosome 12 LUSG00023784,with phenotypic interpretation rates of 12.57 and 11.56％respectively;Two genes were jointly de-tected in the three environments.The NPH3 family gene LUSG00005402 detected at 7626 bp upstream of the SNP(group13:21285679)locus on chromosome 13 and the cell transition metal ion-related gene LUSG0005402 detected at 9888 bp downstream of the SNP(group13:17033610)locus The phenotypic interpretation rates of 04744 were 11.24％and 12.57％respectively.The expression levels of these six candidate genes at the stem and root sites of an-ti-lodging and easily lodging materials were significantly different.It is speculated that these key candidate genes are closely related to the anti-lodging of flax,providing reference value for molecular breeding of anti-lodging of flax.